Exploiting phage display for development of novel cellular targeting strategies

William Marsh, Amanda Witten, Sarah Stabenfeldt

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Targeting strategies for drug delivery applications rely on targeting moieties (i.e., peptide, antibody) specific to the desired cell surface receptor or protein of interest. However, current targeting strategies are limited to previously identified epitopes/ligand pairs. The field of phage display opens up the targeting moiety options whereby new epitope/ligand pairs may be discovered through well-designed biopanning assays for the target cell population of interest. Here, we provide a detailed protocol to perform phage biopanning assays on adherent cell cultures. The methods described here may be modified to user-specific targeting interests.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages71-94
Number of pages24
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1831
ISSN (Print)1064-3745

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Keywords

  • Astrocytes
  • Domain antibody (DAb)
  • Phage display
  • Single chain antibody fragment (ScFv)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Marsh, W., Witten, A., & Stabenfeldt, S. (2018). Exploiting phage display for development of novel cellular targeting strategies. In Methods in Molecular Biology (pp. 71-94). (Methods in Molecular Biology; Vol. 1831). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8661-3_7