Experimental hyperphenylalaninemia

Dendritic alterations in motor cortex of rat

R. N. Hogan, Paul Coleman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Rats were injected i.p. daily from postnatal days 3 through 82 with a combination of the phenylalanine hydroxylase inhibitor p-chlorophenylalanine (60 mg/kg) plus phenylalanine (300 mg/kg), resulting in a significant hyperphenylalaninemia of 5.36 mg 100 ml. Control rats were saline injected. Golgi-Cox-stained layer V pyramidal and layer IV stellate neurons from cortical area 4 were measured using a computerized dendrite tracking system. Basal and oblique dendritic segments of layer V pyramidal cells in animals with hyperphenylalaninemia were fewer in number, shorter, branched closer to the cell soma or apical shaft, and branched less frequently than did dendrites in control animals. Fewer differences were found for layer IV stellate cells. These data suggest that in human hyperphenylalaninemia which is not severe enough to satisfy the classification criterion for phenylketonuria of 16.5 mg 100 ml plasma there may be undetected alterations in the morphology of cortical dendrites.

Original languageEnglish (US)
Pages (from-to)218-233
Number of pages16
JournalExperimental Neurology
Volume74
Issue number1
DOIs
StatePublished - Jan 1 1981
Externally publishedYes

Fingerprint

Phenylketonurias
Motor Cortex
Dendrites
Phenylalanine Hydroxylase
Fenclonine
Pyramidal Cells
Carisoprodol
Phenylalanine
Neurons

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Experimental hyperphenylalaninemia : Dendritic alterations in motor cortex of rat. / Hogan, R. N.; Coleman, Paul.

In: Experimental Neurology, Vol. 74, No. 1, 01.01.1981, p. 218-233.

Research output: Contribution to journalArticle

@article{231d77a1943543088f612dca2dd69c82,
title = "Experimental hyperphenylalaninemia: Dendritic alterations in motor cortex of rat",
abstract = "Rats were injected i.p. daily from postnatal days 3 through 82 with a combination of the phenylalanine hydroxylase inhibitor p-chlorophenylalanine (60 mg/kg) plus phenylalanine (300 mg/kg), resulting in a significant hyperphenylalaninemia of 5.36 mg 100 ml. Control rats were saline injected. Golgi-Cox-stained layer V pyramidal and layer IV stellate neurons from cortical area 4 were measured using a computerized dendrite tracking system. Basal and oblique dendritic segments of layer V pyramidal cells in animals with hyperphenylalaninemia were fewer in number, shorter, branched closer to the cell soma or apical shaft, and branched less frequently than did dendrites in control animals. Fewer differences were found for layer IV stellate cells. These data suggest that in human hyperphenylalaninemia which is not severe enough to satisfy the classification criterion for phenylketonuria of 16.5 mg 100 ml plasma there may be undetected alterations in the morphology of cortical dendrites.",
author = "Hogan, {R. N.} and Paul Coleman",
year = "1981",
month = "1",
day = "1",
doi = "10.1016/0014-4886(81)90160-6",
language = "English (US)",
volume = "74",
pages = "218--233",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Experimental hyperphenylalaninemia

T2 - Dendritic alterations in motor cortex of rat

AU - Hogan, R. N.

AU - Coleman, Paul

PY - 1981/1/1

Y1 - 1981/1/1

N2 - Rats were injected i.p. daily from postnatal days 3 through 82 with a combination of the phenylalanine hydroxylase inhibitor p-chlorophenylalanine (60 mg/kg) plus phenylalanine (300 mg/kg), resulting in a significant hyperphenylalaninemia of 5.36 mg 100 ml. Control rats were saline injected. Golgi-Cox-stained layer V pyramidal and layer IV stellate neurons from cortical area 4 were measured using a computerized dendrite tracking system. Basal and oblique dendritic segments of layer V pyramidal cells in animals with hyperphenylalaninemia were fewer in number, shorter, branched closer to the cell soma or apical shaft, and branched less frequently than did dendrites in control animals. Fewer differences were found for layer IV stellate cells. These data suggest that in human hyperphenylalaninemia which is not severe enough to satisfy the classification criterion for phenylketonuria of 16.5 mg 100 ml plasma there may be undetected alterations in the morphology of cortical dendrites.

AB - Rats were injected i.p. daily from postnatal days 3 through 82 with a combination of the phenylalanine hydroxylase inhibitor p-chlorophenylalanine (60 mg/kg) plus phenylalanine (300 mg/kg), resulting in a significant hyperphenylalaninemia of 5.36 mg 100 ml. Control rats were saline injected. Golgi-Cox-stained layer V pyramidal and layer IV stellate neurons from cortical area 4 were measured using a computerized dendrite tracking system. Basal and oblique dendritic segments of layer V pyramidal cells in animals with hyperphenylalaninemia were fewer in number, shorter, branched closer to the cell soma or apical shaft, and branched less frequently than did dendrites in control animals. Fewer differences were found for layer IV stellate cells. These data suggest that in human hyperphenylalaninemia which is not severe enough to satisfy the classification criterion for phenylketonuria of 16.5 mg 100 ml plasma there may be undetected alterations in the morphology of cortical dendrites.

UR - http://www.scopus.com/inward/record.url?scp=0019486014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019486014&partnerID=8YFLogxK

U2 - 10.1016/0014-4886(81)90160-6

DO - 10.1016/0014-4886(81)90160-6

M3 - Article

VL - 74

SP - 218

EP - 233

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 1

ER -