Excitation dynamics in the core antenna of PS I from Chlamydomonas reinhardtii CC 2696 at room temperature

Krzysztof Gibasiewicz, V. M. Ramesh, Alexander N. Melkozernov, Su Lin, Neal Woodbury, Robert E. Blankenship, Andrew Webber

Research output: Contribution to journalArticle

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Abstract

Photosystem I particles from a eukaryotic organism, the green alga Chlamydomonas reinhardtii CC 2696, were studied by transient hole-burning spectroscopy at room temperature. Global analysis of the spectra recorded after excitation of chlorophyll a molecules in Photosystem I at selected wavelengths between 670 and 710 nm reveals excitation dynamics with subpicosecond, 2-3 ps, and 20-23 ps components. The subpicosecond and 2-3 ps components are ascribed to energy equilibration within the core antenna, whereas the 20-23 ps component is ascribed to energy trapping by the reaction center. Energy equilibration components describe both uphill and downhill energy transfer depending of the excitation wavelength. The initial transient absorbance bands after direct excitation of the red tail of the Qy transition band of chlorophyll a (at 700, 705, and 710 nm) are 25 nm wide and structured, revealing strongly coupled excited states among a group of molecules, most likely reaction center chlorophyll molecules. Excitation at shorter wavelengths (670, 680, and 695 nm) results in only 5-7 nm wide initial absorbance bands originating from photobleaching and stimulated emission of antenna chlorophyll molecules. The results are compared to the excitation dynamics of Photosystem I from the cyanobacterium Synechocystis sp. PCC 6803. The most significant difference is that the 2-3 ps phase describes internal excitation dynamics within higher-energy antenna chlorophyll molecules in the algal PS I system rather than between bulk and red chlorophylls, as observed in cyanobacterial PS I. No indications of core antenna red pigments absorbing above 700 nm were found in the preparation from Chlamydomonas. Independent of excitation wavelength, after at most a few picoseconds, all excitons are distributed over the same pool of chlorophyll molecules centered at ∼682 nm.

Original languageEnglish (US)
Pages (from-to)11498-11506
Number of pages9
JournalJournal of Physical Chemistry B
Volume105
Issue number46
DOIs
StatePublished - Nov 22 2001

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Chlorophyll
chlorophylls
antennas
Antennas
Photosystem I Protein Complex
Molecules
room temperature
excitation
Wavelength
Temperature
molecules
wavelengths
Photobleaching
Stimulated emission
Algae
Excited states
Pigments
Energy transfer
Excitons
energy

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Excitation dynamics in the core antenna of PS I from Chlamydomonas reinhardtii CC 2696 at room temperature. / Gibasiewicz, Krzysztof; Ramesh, V. M.; Melkozernov, Alexander N.; Lin, Su; Woodbury, Neal; Blankenship, Robert E.; Webber, Andrew.

In: Journal of Physical Chemistry B, Vol. 105, No. 46, 22.11.2001, p. 11498-11506.

Research output: Contribution to journalArticle

Gibasiewicz, Krzysztof ; Ramesh, V. M. ; Melkozernov, Alexander N. ; Lin, Su ; Woodbury, Neal ; Blankenship, Robert E. ; Webber, Andrew. / Excitation dynamics in the core antenna of PS I from Chlamydomonas reinhardtii CC 2696 at room temperature. In: Journal of Physical Chemistry B. 2001 ; Vol. 105, No. 46. pp. 11498-11506.
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