Evaluating the spatial and temporal protein production in neural tissue engineering constructs in vitro

Caroline P. Addington, Christine Pauken, Sarah Stabenfeldt

Research output: Contribution to journalArticlepeer-review

Abstract

Tissue engineering constructs are uniquely positioned not only for in vivo regenerative therapeutic applications but also to evaluate cellular responses to modifi cations in fi nely tuned three-dimensional (3-D) microenvironments as opposed to traditional two-dimensional (2-D) culture systems. However, moving into a 3-D system presents complications of assessing spatial and temporal alterations in cellular behavior (e.g., protein synthesis, motility, etc.). Here, we describe methods to evaluate spatial and temporal protein production in neural tissue engineering construct.

Original languageEnglish (US)
Pages (from-to)163-179
Number of pages17
JournalNeuromethods
Volume93
DOIs
StatePublished - Jan 1 2015

Keywords

  • Cryosectioning
  • Immunocytochemistry
  • Neural tissue engineering
  • Western blot

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

Fingerprint Dive into the research topics of 'Evaluating the spatial and temporal protein production in neural tissue engineering constructs in vitro'. Together they form a unique fingerprint.

Cite this