Enzyme-polymer-single walled carbon nanotube composites as biocatalytic films

Kaushal Rege; Nachiket R. Raravikar; Dae Yun Kim; Linda S. Schadler; Pulickel M. Ajayan; Jonathan S. Dordick

doi:10.1021/nl034131k

Enzyme-polymer-single walled carbon nanotube composites as biocatalytic films

Kaushal Rege, Nachiket R. Raravikar, Dae Yun Kim, Linda S. Schadler, Pulickel M. Ajayan, Jonathan S. Dordick

Research output: Contribution to journal › Article › peer-review

161 Scopus citations

Abstract

Enzyme-containing polymer-single walled carbon nanotube (SWNT) composites have been explored as unique biocatalytic materials. The biocatalytic composites were prepared by suspending SWNT and α-chymotrypsin (CT) directly into a poly(methyl methacrylate) solution in toluene. The activity of the resulting CT-polymer-nanotube films was observed to be higher than both polymer-CT and polymer-graphite-CT films. The effect of SWNT loading on biocatalytic activity was also studied, and the optimal SWNT loading was used to compare the biocatalytic performance of different polymer-SWNT systems. In all polymers tested, SWNT-containing composites show higher enzyme activity than the non-SWNT preparations. This was most likely due to a reduction in the leaching of enzyme from the film in the former, perhaps caused by the adsorption of enzyme molecules onto highly structured and high energy SWNTs within the polymer matrix. Investigation of different polymer composites indicates that SWNTs may be used to improve the performance of a wide range of biocatalytic composites for applications ranging from antifouling surface coatings to sensor elements.

Original language	English (US)
Pages (from-to)	829-832
Number of pages	4
Journal	Nano Letters
Volume	3
Issue number	6
DOIs	https://doi.org/10.1021/nl034131k
State	Published - Jun 1 2003
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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title = "Enzyme-polymer-single walled carbon nanotube composites as biocatalytic films",

abstract = "Enzyme-containing polymer-single walled carbon nanotube (SWNT) composites have been explored as unique biocatalytic materials. The biocatalytic composites were prepared by suspending SWNT and α-chymotrypsin (CT) directly into a poly(methyl methacrylate) solution in toluene. The activity of the resulting CT-polymer-nanotube films was observed to be higher than both polymer-CT and polymer-graphite-CT films. The effect of SWNT loading on biocatalytic activity was also studied, and the optimal SWNT loading was used to compare the biocatalytic performance of different polymer-SWNT systems. In all polymers tested, SWNT-containing composites show higher enzyme activity than the non-SWNT preparations. This was most likely due to a reduction in the leaching of enzyme from the film in the former, perhaps caused by the adsorption of enzyme molecules onto highly structured and high energy SWNTs within the polymer matrix. Investigation of different polymer composites indicates that SWNTs may be used to improve the performance of a wide range of biocatalytic composites for applications ranging from antifouling surface coatings to sensor elements.",

author = "Kaushal Rege and Raravikar, {Nachiket R.} and Kim, {Dae Yun} and Schadler, {Linda S.} and Ajayan, {Pulickel M.} and Dordick, {Jonathan S.}",

year = "2003",

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AU - Rege, Kaushal

AU - Raravikar, Nachiket R.

AU - Kim, Dae Yun

AU - Schadler, Linda S.

AU - Ajayan, Pulickel M.

AU - Dordick, Jonathan S.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - Enzyme-containing polymer-single walled carbon nanotube (SWNT) composites have been explored as unique biocatalytic materials. The biocatalytic composites were prepared by suspending SWNT and α-chymotrypsin (CT) directly into a poly(methyl methacrylate) solution in toluene. The activity of the resulting CT-polymer-nanotube films was observed to be higher than both polymer-CT and polymer-graphite-CT films. The effect of SWNT loading on biocatalytic activity was also studied, and the optimal SWNT loading was used to compare the biocatalytic performance of different polymer-SWNT systems. In all polymers tested, SWNT-containing composites show higher enzyme activity than the non-SWNT preparations. This was most likely due to a reduction in the leaching of enzyme from the film in the former, perhaps caused by the adsorption of enzyme molecules onto highly structured and high energy SWNTs within the polymer matrix. Investigation of different polymer composites indicates that SWNTs may be used to improve the performance of a wide range of biocatalytic composites for applications ranging from antifouling surface coatings to sensor elements.

AB - Enzyme-containing polymer-single walled carbon nanotube (SWNT) composites have been explored as unique biocatalytic materials. The biocatalytic composites were prepared by suspending SWNT and α-chymotrypsin (CT) directly into a poly(methyl methacrylate) solution in toluene. The activity of the resulting CT-polymer-nanotube films was observed to be higher than both polymer-CT and polymer-graphite-CT films. The effect of SWNT loading on biocatalytic activity was also studied, and the optimal SWNT loading was used to compare the biocatalytic performance of different polymer-SWNT systems. In all polymers tested, SWNT-containing composites show higher enzyme activity than the non-SWNT preparations. This was most likely due to a reduction in the leaching of enzyme from the film in the former, perhaps caused by the adsorption of enzyme molecules onto highly structured and high energy SWNTs within the polymer matrix. Investigation of different polymer composites indicates that SWNTs may be used to improve the performance of a wide range of biocatalytic composites for applications ranging from antifouling surface coatings to sensor elements.

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Enzyme-polymer-single walled carbon nanotube composites as biocatalytic films

Abstract

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