Endogenously synthesized peptide with an endoplasmic reticulum signal sequence sensitizes antigen processing mutant cells to class i-restricted cell-mediated lysis

K. Anderson, Peter Cresswell, M. Gammon, J. Hermes, A. Williamson, H. Zweerink

Research output: Contribution to journalArticle

196 Scopus citations

Abstract

The HLA-A2-positive human mutant cell line T2 is not lysed by influenza virus-specific HLA A2-restricted cytotoxic lymphocytes after virus infection. However, lysis does occur when cells are incubated with the antigenic influenza matrix protein-derived peptide M57-68. To examine the nature of this defect, T2 cells were transfected with two different plasmids. One plasmid encoded the peptide M57-68, and the other encoded the same peptide preceded by an endoplasmic reticulum translocation signal sequence. Mutant T2 cells expressing the M57-68 peptide without the signal sequence were not susceptible to lysis by M57-68-specific HLAA2-restricted cytotoxic T lymphocytes, whereas T2 cells expressing the M57-68 peptide plus signal sequence were lysed effectively. Lysis of parental T1 cells with either plasmid was equally effective. These results suggest that the T2 mutant cells are defective in the transport of antigenic peptides from the cytosol into the secretory pathway.

Original languageEnglish (US)
Pages (from-to)489-492
Number of pages4
JournalJournal of Experimental Medicine
Volume174
Issue number2
DOIs
StatePublished - Aug 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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