End points of lactate and glucose metabolism after exhausting exercise

G. A. Brooks, Glenn Gaesser

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

To determine the extent of metabolite oxidation, rats were injected with [U-14C]lactate, -glucose, or -bicarbonate (n = 5, each) during rest or after continuous (CE) and intermittent (IE) exercises to exhaustion. Tissue analyses of resting rats, or rats killed following CE and IE and pulse injection with [14C]lactate or -glucose (n = 72, each), were used to determine the metabolic pathways of these two substrates. Oxygen consumption (VO2) declined rapidly for the first 15 min after exercise; thereafter, (VO2) declined slowly and remained elevated above resting levels for 120 min. The slow phase of decline in (VO2) during recovery did not coincide with lactate removal, which occurred within 15 min. Two-dimensional radiochromatograms produced from blood, kidney, liver, skeletal muscle, and heart indicated a rapid incorporation of 14C into several amino acid pools, including alanine, glutamine, glutamate, and aspartate Four-hour postexercise recoveries (means of CE and IE) of injected [14C]lactate were lactate (0.75%), glucose (0.52%), protein (8.57%), glycogen (18.30%), CO2 (45.18%), and HCO- 3 (17.72%). Greater (P < 0.05) incorporation of 14C into protein and glycogen constituents after exercise, compared with rest, was demonstrated. Incorporation of [14C]lactate into glycogen represented a significant but only minor fraction of the metabolism of lactate after exhausting exercise. It is suggested that classical explanations of excess postexercise O2 consumption (i.e., 'O2 debt') are too simplistic.

Original languageEnglish (US)
Pages (from-to)1057-1069
Number of pages13
JournalJournal of Applied Physiology Respiratory Environmental and Exercise Physiology
Volume49
Issue number6
StatePublished - 1980
Externally publishedYes

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Lactic Acid
Glucose
Glycogen
Bicarbonates
Metabolic Networks and Pathways
Glutamine
Aspartic Acid
Oxygen Consumption
Alanine
Glutamic Acid
Skeletal Muscle
Proteins
Kidney
Amino Acids
Injections
Liver

ASJC Scopus subject areas

  • Endocrinology
  • Physiology

Cite this

End points of lactate and glucose metabolism after exhausting exercise. / Brooks, G. A.; Gaesser, Glenn.

In: Journal of Applied Physiology Respiratory Environmental and Exercise Physiology, Vol. 49, No. 6, 1980, p. 1057-1069.

Research output: Contribution to journalArticle

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