14 Citations (Scopus)

Abstract

It has been hypothesized that the ribosome gains additional fidelity during protein translation by probing structural differences in tRNA species. We measure the translocation kinetics of different tRNA species through ∼3 nm diameter synthetic nanopores. Each tRNA species varies in the time scale with which it is deformed from equilibrium, as in the translocation step of protein translation. Using machine-learning algorithms, we can differentiate among five tRNA species, analyze the ratios of tRNA binary mixtures, and distinguish tRNA isoacceptors. (Figure Presented).

Original languageEnglish (US)
Pages (from-to)138-144
Number of pages7
JournalNano Letters
Volume16
Issue number1
DOIs
StatePublished - Jan 13 2016

Fingerprint

ribonucleic acids
Transfer RNA
ribosomes
proteins
machine learning
Molecules
binary mixtures
molecules
kinetics
Proteins
Nanopores
Binary mixtures
Learning algorithms
Learning systems
Kinetics

Keywords

  • nanopores
  • RNA
  • single molecule
  • SVM
  • tRNA

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Henley, R. Y., Ashcroft, B. A., Farrell, I., Cooperman, B. S., Lindsay, S., & Wanunu, M. (2016). Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity. Nano Letters, 16(1), 138-144. https://doi.org/10.1021/acs.nanolett.5b03331

Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity. / Henley, Robert Y.; Ashcroft, Brian Alan; Farrell, Ian; Cooperman, Barry S.; Lindsay, Stuart; Wanunu, Meni.

In: Nano Letters, Vol. 16, No. 1, 13.01.2016, p. 138-144.

Research output: Contribution to journalArticle

Henley, RY, Ashcroft, BA, Farrell, I, Cooperman, BS, Lindsay, S & Wanunu, M 2016, 'Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity', Nano Letters, vol. 16, no. 1, pp. 138-144. https://doi.org/10.1021/acs.nanolett.5b03331
Henley, Robert Y. ; Ashcroft, Brian Alan ; Farrell, Ian ; Cooperman, Barry S. ; Lindsay, Stuart ; Wanunu, Meni. / Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity. In: Nano Letters. 2016 ; Vol. 16, No. 1. pp. 138-144.
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