@article{3d7e0caf05254da4bf5c37bd0c29fed6,
title = "Electrochemically Driven Photosynthetic Electron Transport in Cyanobacteria Lacking Photosystem II",
abstract = "Light-activated photosystem II (PSII) carries out the critical step of splitting water in photosynthesis. However, PSII is susceptible to light-induced damage. Here, results are presented from a novel microbial electro-photosynthetic system (MEPS) that uses redox mediators in conjunction with an electrode to drive electron transport in live Synechocystis (ΔpsbB) cells lacking PSII. MEPS-generated, light-dependent current increased with light intensity up to 2050 μmol photons m-2 s-1, which yielded a delivery rate of 113 μmol electrons h-1 mg-chl-1 and an average current density of 150 A m-2 s-1 mg-chl-1. P700+ re-reduction kinetics demonstrated that initial rates exceeded wildtype PSII-driven electron delivery. The electron delivery occurs ahead of the cytochrome b6f complex to enable both NADPH and ATP production. This work demonstrates an electrochemical system that can drive photosynthetic electron transport, provides a platform for photosynthetic foundational studies, and has the potential for improving photosynthetic performance at high light intensities.",
author = "Lewis, {Christine M.} and Flory, {Justin D.} and Moore, {Thomas A.} and Moore, {Ana L.} and Rittmann, {Bruce E.} and Vermaas, {Wim F.J.} and Torres, {C{\'e}sar I.} and Petra Fromme",
note = "Funding Information: Many thanks to Dr. Michael Vaughn for his expertise and aid in concepts and project layout, to Dr. Yuval Mazor, Dr. Zachary Dobson, Dr. Hila Toperik and Dr. Kevin Redding for methodology of Joliot-type spectroscopy, and to Dr. Chelsea Brown, Dr. Omar Khdour, and Dr. Sydney Hecht (co-PI) for expertise in small molecule delivery of electrons. Additionally, we thank ASU Lightworks for funding and the U.S. Department of Energy, the Biodesign Center for Applied Structural Discovery and Arizona State University School of Molecular Sciences for equipment, instrument use, lab space, supplies and funding and the Biodesign Swette Center for Environmental Biotechnology for supplies, equipment, and instrument use. Funding Information: The initial concept was developed with support from the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under award DE-SC0001016 and was additionally funded by Lightworks at Arizona State University where P.F., J.D.F., C.I.T., B.E.R., W.F.J.V., A.L.M., and T.A.M. were coprincipal investigators and contributed to the original concept which was later patented. A.L.M. and T.A.M. acknowledge support by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Award No. DE-FG02-03ER15393. C.T. would like to acknowledge support by the Office of Naval Research, under Award No. N000141512702. P.F. acknowledges support from the Biodesign Applied Structural Discovery, and the Paul V. Galvin award that provided for summer salaries for C.M.L. Publisher Copyright: {\textcopyright} 2022 The Authors. Published by American Chemical Society",
year = "2022",
month = feb,
day = "23",
doi = "10.1021/jacs.1c09291",
language = "English (US)",
volume = "144",
pages = "2933--2942",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "7",
}