Efficient separation of dolaproine stereoisomers by optimization of a three component chromatographic solvent system

D. Kantoci, George Pettit, T. R. Wardlaw

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A solvent system composed of n-hexane-chloroform-acetone was selected (by TLC) for resolving a difficult to separate mixture of protected dolaproine stereoisomers. A set of points representing solvent ratios was generated by an application of statistical methods. From thin layer chromatography data semifinal solvent ratios were selected. By applying simplex optimization, the solvent ratio was found to be 32.3 : 53.4 : 1.43 respectively for the preceding solvent system.

Original languageEnglish (US)
Pages (from-to)3915-3923
Number of pages9
JournalJournal of Liquid Chromatography
Issue number20
Publication statusPublished - 1990


ASJC Scopus subject areas

  • Molecular Medicine

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