A solvent system composed of n-hexane-chloroform-acetone was selected (by TLC) for resolving a difficult to separate mixture of protected dolaproine stereoisomers. A set of points representing solvent ratios was generated by an application of statistical methods. From thin layer chromatography data semifinal solvent ratios were selected. By applying simplex optimization, the solvent ratio was found to be 32.3 : 53.4 : 14.3 respectively for the preceding solvent system.
ASJC Scopus subject areas
- Molecular Medicine