Easily fabricated magnetic traps for single-cell applications

John H. Koschwanez; Robert H. Carlson; Deirdre R. Meldrum

doi:10.1063/1.2722400

Easily fabricated magnetic traps for single-cell applications

John H. Koschwanez, Robert H. Carlson, Deirdre R. Meldrum

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl2 catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.

Original language	English (US)
Article number	044301
Journal	Review of Scientific Instruments
Volume	78
Issue number	4
DOIs	https://doi.org/10.1063/1.2722400
State	Published - 2007
Externally published	Yes

ASJC Scopus subject areas

Instrumentation

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10.1063/1.2722400

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title = "Easily fabricated magnetic traps for single-cell applications",

abstract = "We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl2 catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.",

author = "Koschwanez, {John H.} and Carlson, {Robert H.} and Meldrum, {Deirdre R.}",

note = "Funding Information: The authors thank the NanoTech User Facility at the University of Washington for use of the AFM and the SEM, Barry Lutz for assistance with microfluidic flow characterization, Daniel Gottschling and Michael McMurray for assistance with yeast biology, and NIH NHGRI Grant No. 1 R01 HG01497 for funding.",

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doi = "10.1063/1.2722400",

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AU - Koschwanez, John H.

AU - Carlson, Robert H.

AU - Meldrum, Deirdre R.

N1 - Funding Information: The authors thank the NanoTech User Facility at the University of Washington for use of the AFM and the SEM, Barry Lutz for assistance with microfluidic flow characterization, Daniel Gottschling and Michael McMurray for assistance with yeast biology, and NIH NHGRI Grant No. 1 R01 HG01497 for funding.

PY - 2007

Y1 - 2007

N2 - We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl2 catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.

AB - We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl2 catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.

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Easily fabricated magnetic traps for single-cell applications

Abstract

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