Easily fabricated magnetic traps for single-cell applications

John H. Koschwanez, Robert H. Carlson, Deirdre Meldrum

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl2 catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.

Original languageEnglish (US)
Article number044301
JournalReview of Scientific Instruments
Volume78
Issue number4
DOIs
StatePublished - 2007
Externally publishedYes

Fingerprint

Polydimethylsiloxane
Microfluidics
Yeast
Cells
traps
Catalysts
yeast
cells
Foams
Plasmas
catalysts
microfluidic devices
foams
Temperature
estimates
temperature

ASJC Scopus subject areas

  • Instrumentation
  • Physics and Astronomy (miscellaneous)

Cite this

Easily fabricated magnetic traps for single-cell applications. / Koschwanez, John H.; Carlson, Robert H.; Meldrum, Deirdre.

In: Review of Scientific Instruments, Vol. 78, No. 4, 044301, 2007.

Research output: Contribution to journalArticle

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