Easily fabricated magnetic traps for single-cell applications

John H. Koschwanez, Robert H. Carlson, Deirdre R. Meldrum

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl2 catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.

Original languageEnglish (US)
Article number044301
JournalReview of Scientific Instruments
Volume78
Issue number4
DOIs
StatePublished - May 8 2007
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Instrumentation

Cite this