Double-flow focused liquid injector for efficient serial femtosecond crystallography

Dominik Oberthuer, Juraj Knoška, Max O. Wiedorn, Kenneth R. Beyerlein, David A. Bushnell, Elena G. Kovaleva, Michael Heymann, Lars Gumprecht, Richard Kirian, Anton Barty, Valerio Mariani, Aleksandra Tolstikova, Luigi Adriano, Salah Awel, Miriam Barthelmess, Katerina Dörner, P. Lourdu Xavier, Oleksandr Yefanov, Daniel R. James, Garrett Nelson & 25 others Dingjie Wang, George Calvey, Yujie Chen, Andrea Schmidt, Michael Szczepek, Stefan Frielingsdorf, Oliver Lenz, Edward Snell, Philip J. Robinson, Böidar Šarler, Grega Belšak, Marjan Maček, Fabian Wilde, Andrew Aquila, Sébastien Boutet, Mengning Liang, Mark S. Hunter, Patrick Scheerer, John D. Lipscomb, Uwe Weierstall, Roger D. Kornberg, John Spence, Lois Pollack, Henry N. Chapman, Saša Bajt

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

    Original languageEnglish (US)
    Article number44628
    JournalScientific Reports
    Volume7
    DOIs
    StatePublished - Mar 16 2017

    Fingerprint

    liquid flow
    injectors
    nozzles
    crystallography
    room temperature
    free electron lasers
    delivery
    proteins
    rings
    high resolution
    crystals
    x rays

    ASJC Scopus subject areas

    • General

    Cite this

    Oberthuer, D., Knoška, J., Wiedorn, M. O., Beyerlein, K. R., Bushnell, D. A., Kovaleva, E. G., ... Bajt, S. (2017). Double-flow focused liquid injector for efficient serial femtosecond crystallography. Scientific Reports, 7, [44628]. https://doi.org/10.1038/srep44628

    Double-flow focused liquid injector for efficient serial femtosecond crystallography. / Oberthuer, Dominik; Knoška, Juraj; Wiedorn, Max O.; Beyerlein, Kenneth R.; Bushnell, David A.; Kovaleva, Elena G.; Heymann, Michael; Gumprecht, Lars; Kirian, Richard; Barty, Anton; Mariani, Valerio; Tolstikova, Aleksandra; Adriano, Luigi; Awel, Salah; Barthelmess, Miriam; Dörner, Katerina; Xavier, P. Lourdu; Yefanov, Oleksandr; James, Daniel R.; Nelson, Garrett; Wang, Dingjie; Calvey, George; Chen, Yujie; Schmidt, Andrea; Szczepek, Michael; Frielingsdorf, Stefan; Lenz, Oliver; Snell, Edward; Robinson, Philip J.; Šarler, Böidar; Belšak, Grega; Maček, Marjan; Wilde, Fabian; Aquila, Andrew; Boutet, Sébastien; Liang, Mengning; Hunter, Mark S.; Scheerer, Patrick; Lipscomb, John D.; Weierstall, Uwe; Kornberg, Roger D.; Spence, John; Pollack, Lois; Chapman, Henry N.; Bajt, Saša.

    In: Scientific Reports, Vol. 7, 44628, 16.03.2017.

    Research output: Contribution to journalArticle

    Oberthuer, D, Knoška, J, Wiedorn, MO, Beyerlein, KR, Bushnell, DA, Kovaleva, EG, Heymann, M, Gumprecht, L, Kirian, R, Barty, A, Mariani, V, Tolstikova, A, Adriano, L, Awel, S, Barthelmess, M, Dörner, K, Xavier, PL, Yefanov, O, James, DR, Nelson, G, Wang, D, Calvey, G, Chen, Y, Schmidt, A, Szczepek, M, Frielingsdorf, S, Lenz, O, Snell, E, Robinson, PJ, Šarler, B, Belšak, G, Maček, M, Wilde, F, Aquila, A, Boutet, S, Liang, M, Hunter, MS, Scheerer, P, Lipscomb, JD, Weierstall, U, Kornberg, RD, Spence, J, Pollack, L, Chapman, HN & Bajt, S 2017, 'Double-flow focused liquid injector for efficient serial femtosecond crystallography' Scientific Reports, vol. 7, 44628. https://doi.org/10.1038/srep44628
    Oberthuer D, Knoška J, Wiedorn MO, Beyerlein KR, Bushnell DA, Kovaleva EG et al. Double-flow focused liquid injector for efficient serial femtosecond crystallography. Scientific Reports. 2017 Mar 16;7. 44628. https://doi.org/10.1038/srep44628
    Oberthuer, Dominik ; Knoška, Juraj ; Wiedorn, Max O. ; Beyerlein, Kenneth R. ; Bushnell, David A. ; Kovaleva, Elena G. ; Heymann, Michael ; Gumprecht, Lars ; Kirian, Richard ; Barty, Anton ; Mariani, Valerio ; Tolstikova, Aleksandra ; Adriano, Luigi ; Awel, Salah ; Barthelmess, Miriam ; Dörner, Katerina ; Xavier, P. Lourdu ; Yefanov, Oleksandr ; James, Daniel R. ; Nelson, Garrett ; Wang, Dingjie ; Calvey, George ; Chen, Yujie ; Schmidt, Andrea ; Szczepek, Michael ; Frielingsdorf, Stefan ; Lenz, Oliver ; Snell, Edward ; Robinson, Philip J. ; Šarler, Böidar ; Belšak, Grega ; Maček, Marjan ; Wilde, Fabian ; Aquila, Andrew ; Boutet, Sébastien ; Liang, Mengning ; Hunter, Mark S. ; Scheerer, Patrick ; Lipscomb, John D. ; Weierstall, Uwe ; Kornberg, Roger D. ; Spence, John ; Pollack, Lois ; Chapman, Henry N. ; Bajt, Saša. / Double-flow focused liquid injector for efficient serial femtosecond crystallography. In: Scientific Reports. 2017 ; Vol. 7.
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    AU - Kovaleva, Elena G.

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    AU - Adriano, Luigi

    AU - Awel, Salah

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    AU - Frielingsdorf, Stefan

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    AU - Lipscomb, John D.

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