Dot immunofiltration assay for quantitative detecting cTnI using quantum dots

Jia Fan, Jian Song, Li Rong Bi, Guang Yu Zhou, Hao Zhang, Jing Yan Wei, Bai Yang

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Quantum dots have physical and optical properties that make them useful tools for high-resolution labeling immunoassay. In this work, a rapid and simple method of quantitative immunoassay for Cardiac troponin I(cTnI) was developed with quantum dots-labeled antibodies. The monoclonal antibodies of cTnI(2F11) could be labeled with CdTe quantum dots and the coupled product(CdTe-2F11) were characterized by SDS-PAGE. The result of immunofiltration assay indicats that the CdTe-2F11 maintains the antibody activity. The cTnI at the different concentrations in NC membrane could react with CdTe-2F11 and be detected with Image Master to analyze the fluorescence intensity of the immunodotting. The results show that the detection limit of cTnI is 120 ng, and there is a good linear relation between concentration of cTnI and the fluorescence intensity(R2=0.9966).

Original languageEnglish (US)
Pages (from-to)2180-2183
Number of pages4
JournalGaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities
Volume30
Issue number11
StatePublished - Nov 1 2009

Keywords

  • Cardiac troponin I
  • Dot immunofiltration assay
  • Monoclonal antibody to cardiac troponin I
  • Quantum dot

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Dot immunofiltration assay for quantitative detecting cTnI using quantum dots'. Together they form a unique fingerprint.

  • Cite this

    Fan, J., Song, J., Bi, L. R., Zhou, G. Y., Zhang, H., Wei, J. Y., & Yang, B. (2009). Dot immunofiltration assay for quantitative detecting cTnI using quantum dots. Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities, 30(11), 2180-2183.