Dot immunofiltration assay for quantitative detecting cTnI using quantum dots

Jia Fan; Jian Song; Li Rong Bi; Guang Yu Zhou; Hao Zhang; Jing Yan Wei; Bai Yang

Dot immunofiltration assay for quantitative detecting cTnI using quantum dots

Jia Fan, Jian Song, Li Rong Bi, Guang Yu Zhou, Hao Zhang, Jing Yan Wei, Bai Yang

Research output: Contribution to journal › Article › peer-review

Abstract

Quantum dots have physical and optical properties that make them useful tools for high-resolution labeling immunoassay. In this work, a rapid and simple method of quantitative immunoassay for Cardiac troponin I(cTnI) was developed with quantum dots-labeled antibodies. The monoclonal antibodies of cTnI(2F11) could be labeled with CdTe quantum dots and the coupled product(CdTe-2F11) were characterized by SDS-PAGE. The result of immunofiltration assay indicats that the CdTe-2F11 maintains the antibody activity. The cTnI at the different concentrations in NC membrane could react with CdTe-2F11 and be detected with Image Master to analyze the fluorescence intensity of the immunodotting. The results show that the detection limit of cTnI is 120 ng, and there is a good linear relation between concentration of cTnI and the fluorescence intensity(R²=0.9966).

Original language	English (US)
Pages (from-to)	2180-2183
Number of pages	4
Journal	Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities
Volume	30
Issue number	11
State	Published - Nov 2009
Externally published	Yes

Keywords

Cardiac troponin I
Dot immunofiltration assay
Monoclonal antibody to cardiac troponin I
Quantum dot

ASJC Scopus subject areas

General Chemistry

Cite this

@article{5f011bd85c09408e8f7a9ad3c99fa382,

title = "Dot immunofiltration assay for quantitative detecting cTnI using quantum dots",

abstract = "Quantum dots have physical and optical properties that make them useful tools for high-resolution labeling immunoassay. In this work, a rapid and simple method of quantitative immunoassay for Cardiac troponin I(cTnI) was developed with quantum dots-labeled antibodies. The monoclonal antibodies of cTnI(2F11) could be labeled with CdTe quantum dots and the coupled product(CdTe-2F11) were characterized by SDS-PAGE. The result of immunofiltration assay indicats that the CdTe-2F11 maintains the antibody activity. The cTnI at the different concentrations in NC membrane could react with CdTe-2F11 and be detected with Image Master to analyze the fluorescence intensity of the immunodotting. The results show that the detection limit of cTnI is 120 ng, and there is a good linear relation between concentration of cTnI and the fluorescence intensity(R2=0.9966).",

keywords = "Cardiac troponin I, Dot immunofiltration assay, Monoclonal antibody to cardiac troponin I, Quantum dot",

author = "Jia Fan and Jian Song and Bi, {Li Rong} and Zhou, {Guang Yu} and Hao Zhang and Wei, {Jing Yan} and Bai Yang",

year = "2009",

month = nov,

language = "English (US)",

volume = "30",

pages = "2180--2183",

journal = "Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities",

issn = "0251-0790",

publisher = "Higher Education Press",

number = "11",

}

TY - JOUR

T1 - Dot immunofiltration assay for quantitative detecting cTnI using quantum dots

AU - Fan, Jia

AU - Song, Jian

AU - Bi, Li Rong

AU - Zhou, Guang Yu

AU - Zhang, Hao

AU - Wei, Jing Yan

AU - Yang, Bai

PY - 2009/11

Y1 - 2009/11

N2 - Quantum dots have physical and optical properties that make them useful tools for high-resolution labeling immunoassay. In this work, a rapid and simple method of quantitative immunoassay for Cardiac troponin I(cTnI) was developed with quantum dots-labeled antibodies. The monoclonal antibodies of cTnI(2F11) could be labeled with CdTe quantum dots and the coupled product(CdTe-2F11) were characterized by SDS-PAGE. The result of immunofiltration assay indicats that the CdTe-2F11 maintains the antibody activity. The cTnI at the different concentrations in NC membrane could react with CdTe-2F11 and be detected with Image Master to analyze the fluorescence intensity of the immunodotting. The results show that the detection limit of cTnI is 120 ng, and there is a good linear relation between concentration of cTnI and the fluorescence intensity(R2=0.9966).

AB - Quantum dots have physical and optical properties that make them useful tools for high-resolution labeling immunoassay. In this work, a rapid and simple method of quantitative immunoassay for Cardiac troponin I(cTnI) was developed with quantum dots-labeled antibodies. The monoclonal antibodies of cTnI(2F11) could be labeled with CdTe quantum dots and the coupled product(CdTe-2F11) were characterized by SDS-PAGE. The result of immunofiltration assay indicats that the CdTe-2F11 maintains the antibody activity. The cTnI at the different concentrations in NC membrane could react with CdTe-2F11 and be detected with Image Master to analyze the fluorescence intensity of the immunodotting. The results show that the detection limit of cTnI is 120 ng, and there is a good linear relation between concentration of cTnI and the fluorescence intensity(R2=0.9966).

KW - Cardiac troponin I

KW - Dot immunofiltration assay

KW - Monoclonal antibody to cardiac troponin I

KW - Quantum dot

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SN - 0251-0790

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JO - Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities

JF - Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities

IS - 11

ER -

Dot immunofiltration assay for quantitative detecting cTnI using quantum dots

Abstract

Keywords

ASJC Scopus subject areas

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