Abstract
Bioassay-guided fractionation of an active methyl ethyl ketone extract of Tetracera boiviniana, using a sensitive assay to monitor DNA polymerase β inhibition, resulted in the isolation of three known triterpenoids, betulinic acid (1), 3- cis-p-coumaroyl maslinic acid (2), and 3-trans-p-coumaroyl maslinic acid (3). Compounds 1-3 inhibited DNA polymerase β with IC50 values of 14, 15, and 4.2 μM in the presence of bovine serum albumin (BSA) and 6.5, 7.5, and 2.0 μM in the absence of BSA, respectively. Further, compounds 1-3 potentiated the effects of bleomycin in cultured P-388D1 cells.
Original language | English (US) |
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Pages (from-to) | 1660-1663 |
Number of pages | 4 |
Journal | Journal of Natural Products |
Volume | 62 |
Issue number | 12 |
DOIs | |
State | Published - Dec 1999 |
Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry
- Molecular Medicine
- Pharmacology
- Pharmaceutical Science
- Drug Discovery
- Complementary and alternative medicine
- Organic Chemistry