DNA origami nanostructures can exhibit preferential renal uptake and alleviate acute kidney injury

Dawei Jiang, Zhilei Ge, Hyung Jun Im, Christopher G. England, Dalong Ni, Junjun Hou, Luhao Zhang, Christopher J. Kutyreff, Yongjun Yan, Yan Liu, Steve Y. Cho, Jonathan W. Engle, Jiye Shi, Peng Huang, Chunhai Fan, Hao Yan, Weibo Cai

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Patients with acute kidney injury (AKI) frequently require kidney transplantation and supportive therapies, such as rehydration and dialysis. Here, we show that radiolabelled DNA origami nanostructures (DONs) with rectangular, triangular and tubular shapes accumulate preferentially in the kidneys of healthy mice and mice with rhabdomyolysis-induced AKI, and that rectangular DONs have renal-protective properties, with efficacy similar to the antioxidant N-acetylcysteine—a clinically used drug that ameliorates contrast-induced AKI and protects kidney function from nephrotoxic agents. We evaluated the biodistribution of DONs non-invasively via positron emission tomography, and the efficacy of rectangular DONs in the treatment of AKI via dynamic positron emission tomography imaging with 68Ga-EDTA, blood tests and kidney tissue staining. DNA-based nanostructures could become a source of therapeutic agents for the treatment of AKI and other renal diseases.

Original languageEnglish (US)
Pages (from-to)865-877
Number of pages13
JournalNature Biomedical Engineering
Volume2
Issue number11
DOIs
StatePublished - Nov 1 2018

Fingerprint

Nanostructures
Acute Kidney Injury
DNA
Kidney
Positron emission tomography
Positron-Emission Tomography
Rhabdomyolysis
Dialysis
Fluid Therapy
Ethylenediaminetetraacetic acid
Hematologic Tests
Therapeutics
Antioxidants
Edetic Acid
Kidney Transplantation
Blood
Tissue
Staining and Labeling
Imaging techniques
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Computer Science Applications

Cite this

DNA origami nanostructures can exhibit preferential renal uptake and alleviate acute kidney injury. / Jiang, Dawei; Ge, Zhilei; Im, Hyung Jun; England, Christopher G.; Ni, Dalong; Hou, Junjun; Zhang, Luhao; Kutyreff, Christopher J.; Yan, Yongjun; Liu, Yan; Cho, Steve Y.; Engle, Jonathan W.; Shi, Jiye; Huang, Peng; Fan, Chunhai; Yan, Hao; Cai, Weibo.

In: Nature Biomedical Engineering, Vol. 2, No. 11, 01.11.2018, p. 865-877.

Research output: Contribution to journalArticle

Jiang, D, Ge, Z, Im, HJ, England, CG, Ni, D, Hou, J, Zhang, L, Kutyreff, CJ, Yan, Y, Liu, Y, Cho, SY, Engle, JW, Shi, J, Huang, P, Fan, C, Yan, H & Cai, W 2018, 'DNA origami nanostructures can exhibit preferential renal uptake and alleviate acute kidney injury', Nature Biomedical Engineering, vol. 2, no. 11, pp. 865-877. https://doi.org/10.1038/s41551-018-0317-8
Jiang, Dawei ; Ge, Zhilei ; Im, Hyung Jun ; England, Christopher G. ; Ni, Dalong ; Hou, Junjun ; Zhang, Luhao ; Kutyreff, Christopher J. ; Yan, Yongjun ; Liu, Yan ; Cho, Steve Y. ; Engle, Jonathan W. ; Shi, Jiye ; Huang, Peng ; Fan, Chunhai ; Yan, Hao ; Cai, Weibo. / DNA origami nanostructures can exhibit preferential renal uptake and alleviate acute kidney injury. In: Nature Biomedical Engineering. 2018 ; Vol. 2, No. 11. pp. 865-877.
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