DNA barcoding of chlorarachniophytes using nucleomorph its sequences

Gillian Gile, Rowena F. Stern, Erick R. James, Patrick J. Keeling

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Chlorarachniophytes are a small group of marine photosynthetic protists. They are best known as examples of an intermediate stage of secondary endosymbiosis: their plastids are derived from green algae and retain a highly reduced nucleus, called a nucleomorph, between the inner and outer pairs of membranes. Chlorarachniophytes can be challenging to identify to the species level, due to their small size, complex life cycles, and the fact that even genus-level diagnostic morphological characters are observable only by EM. Few species have been formally described, and many available culture collection strains remain unnamed. To alleviate this difficulty, we have developed a barcoding system for rapid and accurate identification of chlorarachniophyte species in culture, based on the internal transcribed spacer (ITS) region of the nucleomorph rRNA cistron. Although this is a multicopy locus, encoded in both subtelomeric regions of each chromosome, interlocus variability is low due to gene conversion by homologous recombination in this region. Here, we present barcode sequences for 39 cultured strains of chlorarachniophytes (>80% of currently available strains). Based on barcode data, other published molecular data, and information from culture records, we were able to recommend names for 21 out of the 24 unidentified, partially identified, or misidentified chlorarachniophyte strains in culture. Most strains could be assigned to previously described species, but at least two to as many as five new species may be present among cultured strains.

Original languageEnglish (US)
Pages (from-to)743-750
Number of pages8
JournalJournal of Phycology
Volume46
Issue number4
DOIs
StatePublished - Aug 2010
Externally publishedYes

Fingerprint

DNA barcoding
DNA
barcoding
protist
plastid
green alga
recombination
chromosome
gene conversion
life cycle
homologous recombination
new species
membrane
symbiosis
internal transcribed spacers
plastids
Chlorophyta
gene
life cycle (organisms)
ribosomal RNA

Keywords

  • Bigelowiella
  • Chlorarachnion
  • Culture collections
  • Gymnochlora
  • Internal transcribed spacer
  • Lotharella
  • Norrisiella
  • Partenskyella

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

DNA barcoding of chlorarachniophytes using nucleomorph its sequences. / Gile, Gillian; Stern, Rowena F.; James, Erick R.; Keeling, Patrick J.

In: Journal of Phycology, Vol. 46, No. 4, 08.2010, p. 743-750.

Research output: Contribution to journalArticle

Gile, Gillian ; Stern, Rowena F. ; James, Erick R. ; Keeling, Patrick J. / DNA barcoding of chlorarachniophytes using nucleomorph its sequences. In: Journal of Phycology. 2010 ; Vol. 46, No. 4. pp. 743-750.
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