Diversity of Beet curly top Iran virus isolated from different hosts in Iran

Sara Gharouni Kardani, Jahangir Heydarnejad, Mohammad Zakiaghl, Mohsen Mehrvar, Simona Kraberger, Arvind Varsani

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Beet curly top Iran virus (BCTIV) is a major pathogen of sugar beet in Iran. In order to study diversity of BCTIV, we sampled 68 plants in Iran during the summer of 2010 with curly top disease symptoms on beans (Phaseolus vulgaris), cowpeas (Vigna unguiculata), tomatoes (Solanum lycopersicum L.), sea beets (Beta vulgaris subsp. maritima), and sugar beets (Beta vulgaris). Plant samples showing leaf curling, yellowing, and/or swelling of veins on the lower leaf surfaces were collected from various fields in Khorasan Razavi, Northern Khorasan (north-eastern Iran), East Azarbayejan, West Azarbayejan (north-western Iran), and Fars (southern Iran) provinces. Using rolling circle amplification coupled with restriction digests, cloning, and Sanger sequencing, we determined the genomes of nine new BCTIV isolates from bean, cowpea, tomato, sea beet, and sugar beet in Iran. Our analysis reveals ~11 % diversity amongst BCTIV isolates and we detect evidence of recombination within these genomes.

Original languageEnglish (US)
Pages (from-to)571-575
Number of pages5
JournalVirus Genes
Volume46
Issue number3
DOIs
StatePublished - Jun 1 2013
Externally publishedYes

Keywords

  • Bean
  • Beet curly top Iran virus
  • Cowpea
  • Genetic variability
  • Iran
  • Rolling circle amplification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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