Abstract
Our understanding of the diversity and abundance of circular replication associated protein (Rep) - encoding single stranded (CRESS) DNA viruses has increased considerably over the last few years due to a combination of modern sequencing technologies and new molecular tools. Studies have used these to identify and recover CRESS DNA viruses from a range of different marine organisms, including copepods, shrimp and molluscs. In our study we identified 79 novel CRESS DNA viruses from three mollusc species (Austrovenus stutchburyi, Paphies subtriangulata and Amphibola crenata) and benthic sediments from the Avon-Heathcote estuary in Christchurch, New Zealand. The genomes recovered have varying genome architectures, with all encoding at least two major ORFs that have either unidirectional or bidirectional organisation. Analysis of the Reps of the viral genomes showed they are all highly diverse, with only one Rep sequence sharing 65% amino acid identity with the Rep of gastropod-associated circular DNA virus (GaCSV). Our study adds significantly to the wealth of CRESS DNA viruses recovered from freshwater and marine environments and extends our knowledge of the distribution of these viruses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 284-295 |
| Number of pages | 12 |
| Journal | Infection, Genetics and Evolution |
| Volume | 31 |
| DOIs | |
| State | Published - Apr 1 2015 |
| Externally published | Yes |
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Keywords
- Amphibola crenata
- Austrovenus stutchburyi
- Circular DNA viruses
- CRESS DNA viruses
- Molluscs
- Paphies subtriangulata
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Molecular Biology
- Microbiology
- Infectious Diseases
- Microbiology (medical)
- Medicine(all)
Cite this
Diverse small circular DNA viruses circulating amongst estuarine molluscs. / Dayaram, Anisha; Goldstien, Sharyn; Argüello-Astorga, Gerardo R.; Zawar-Reza, Peyman; Gomez, Christopher; Harding, Jon S.; Varsani, Arvind.
In: Infection, Genetics and Evolution, Vol. 31, 01.04.2015, p. 284-295.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Diverse small circular DNA viruses circulating amongst estuarine molluscs
AU - Dayaram, Anisha
AU - Goldstien, Sharyn
AU - Argüello-Astorga, Gerardo R.
AU - Zawar-Reza, Peyman
AU - Gomez, Christopher
AU - Harding, Jon S.
AU - Varsani, Arvind
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Our understanding of the diversity and abundance of circular replication associated protein (Rep) - encoding single stranded (CRESS) DNA viruses has increased considerably over the last few years due to a combination of modern sequencing technologies and new molecular tools. Studies have used these to identify and recover CRESS DNA viruses from a range of different marine organisms, including copepods, shrimp and molluscs. In our study we identified 79 novel CRESS DNA viruses from three mollusc species (Austrovenus stutchburyi, Paphies subtriangulata and Amphibola crenata) and benthic sediments from the Avon-Heathcote estuary in Christchurch, New Zealand. The genomes recovered have varying genome architectures, with all encoding at least two major ORFs that have either unidirectional or bidirectional organisation. Analysis of the Reps of the viral genomes showed they are all highly diverse, with only one Rep sequence sharing 65% amino acid identity with the Rep of gastropod-associated circular DNA virus (GaCSV). Our study adds significantly to the wealth of CRESS DNA viruses recovered from freshwater and marine environments and extends our knowledge of the distribution of these viruses.
AB - Our understanding of the diversity and abundance of circular replication associated protein (Rep) - encoding single stranded (CRESS) DNA viruses has increased considerably over the last few years due to a combination of modern sequencing technologies and new molecular tools. Studies have used these to identify and recover CRESS DNA viruses from a range of different marine organisms, including copepods, shrimp and molluscs. In our study we identified 79 novel CRESS DNA viruses from three mollusc species (Austrovenus stutchburyi, Paphies subtriangulata and Amphibola crenata) and benthic sediments from the Avon-Heathcote estuary in Christchurch, New Zealand. The genomes recovered have varying genome architectures, with all encoding at least two major ORFs that have either unidirectional or bidirectional organisation. Analysis of the Reps of the viral genomes showed they are all highly diverse, with only one Rep sequence sharing 65% amino acid identity with the Rep of gastropod-associated circular DNA virus (GaCSV). Our study adds significantly to the wealth of CRESS DNA viruses recovered from freshwater and marine environments and extends our knowledge of the distribution of these viruses.
KW - Amphibola crenata
KW - Austrovenus stutchburyi
KW - Circular DNA viruses
KW - CRESS DNA viruses
KW - Molluscs
KW - Paphies subtriangulata
UR - http://www.scopus.com/inward/record.url?scp=84923357388&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84923357388&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2015.02.010
DO - 10.1016/j.meegid.2015.02.010
M3 - Article
C2 - 25697886
AN - SCOPUS:84923357388
VL - 31
SP - 284
EP - 295
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
ER -