Diverse small circular DNA viruses circulating amongst estuarine molluscs

Anisha Dayaram, Sharyn Goldstien, Gerardo R. Argüello-Astorga, Peyman Zawar-Reza, Christopher Gomez, Jon S. Harding, Arvind Varsani

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Our understanding of the diversity and abundance of circular replication associated protein (Rep) - encoding single stranded (CRESS) DNA viruses has increased considerably over the last few years due to a combination of modern sequencing technologies and new molecular tools. Studies have used these to identify and recover CRESS DNA viruses from a range of different marine organisms, including copepods, shrimp and molluscs. In our study we identified 79 novel CRESS DNA viruses from three mollusc species (Austrovenus stutchburyi, Paphies subtriangulata and Amphibola crenata) and benthic sediments from the Avon-Heathcote estuary in Christchurch, New Zealand. The genomes recovered have varying genome architectures, with all encoding at least two major ORFs that have either unidirectional or bidirectional organisation. Analysis of the Reps of the viral genomes showed they are all highly diverse, with only one Rep sequence sharing 65% amino acid identity with the Rep of gastropod-associated circular DNA virus (GaCSV). Our study adds significantly to the wealth of CRESS DNA viruses recovered from freshwater and marine environments and extends our knowledge of the distribution of these viruses.

Original languageEnglish (US)
Pages (from-to)284-295
Number of pages12
JournalInfection, Genetics and Evolution
Volume31
DOIs
StatePublished - Apr 1 2015
Externally publishedYes

Fingerprint

circular DNA
Circular DNA
DNA viruses
DNA Viruses
Mollusca
mollusc
molluscs
virus
DNA
genome
ssDNA viruses
protein
Genome
Copepoda
Estuaries
Aquatic Organisms
Proteins
Gastropoda
marine environment
Viral Genome

Keywords

  • Amphibola crenata
  • Austrovenus stutchburyi
  • Circular DNA viruses
  • CRESS DNA viruses
  • Molluscs
  • Paphies subtriangulata

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)
  • Medicine(all)

Cite this

Dayaram, A., Goldstien, S., Argüello-Astorga, G. R., Zawar-Reza, P., Gomez, C., Harding, J. S., & Varsani, A. (2015). Diverse small circular DNA viruses circulating amongst estuarine molluscs. Infection, Genetics and Evolution, 31, 284-295. https://doi.org/10.1016/j.meegid.2015.02.010

Diverse small circular DNA viruses circulating amongst estuarine molluscs. / Dayaram, Anisha; Goldstien, Sharyn; Argüello-Astorga, Gerardo R.; Zawar-Reza, Peyman; Gomez, Christopher; Harding, Jon S.; Varsani, Arvind.

In: Infection, Genetics and Evolution, Vol. 31, 01.04.2015, p. 284-295.

Research output: Contribution to journalArticle

Dayaram, A, Goldstien, S, Argüello-Astorga, GR, Zawar-Reza, P, Gomez, C, Harding, JS & Varsani, A 2015, 'Diverse small circular DNA viruses circulating amongst estuarine molluscs', Infection, Genetics and Evolution, vol. 31, pp. 284-295. https://doi.org/10.1016/j.meegid.2015.02.010
Dayaram A, Goldstien S, Argüello-Astorga GR, Zawar-Reza P, Gomez C, Harding JS et al. Diverse small circular DNA viruses circulating amongst estuarine molluscs. Infection, Genetics and Evolution. 2015 Apr 1;31:284-295. https://doi.org/10.1016/j.meegid.2015.02.010
Dayaram, Anisha ; Goldstien, Sharyn ; Argüello-Astorga, Gerardo R. ; Zawar-Reza, Peyman ; Gomez, Christopher ; Harding, Jon S. ; Varsani, Arvind. / Diverse small circular DNA viruses circulating amongst estuarine molluscs. In: Infection, Genetics and Evolution. 2015 ; Vol. 31. pp. 284-295.
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