Distribution and phylogeny of EFL and EF-1α in Euglenozoa Suggest ancestral co-occurrence followed by differential loss

Gillian Gile, Drahomíra Faktorová, Christina A. Castlejohn, Gertraud Burger, B. Franz Lang, Mark A. Farmer, Julius Lukeš, Patrick J. Keeling

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: The eukaryotic elongation factor EF-1α (also known as EF1A) catalyzes aminoacyl-tRNA binding by the ribosome during translation. Homologs of this essential protein occur in all domains of life, and it was previously thought to be ubiquitous in eukaryotes. Recently, however, a number of eukaryotes were found to lack EF-1α and instead encode a related protein called EFL (for EF-Like). EFL-encoding organisms are scattered widely across the tree of eukaryotes, and all have close relatives that encode EF-1α. This intriguingly complex distribution has been attributed to multiple lateral transfers because EFL's near mutual exclusivity with EF-1α makes an extended period of co-occurrence seem unlikely. However, differential loss may play a role in EFL evolution, and this possibility has been less widely discussed. Methodology/Principal Findings: We have undertaken an EST- and PCR-based survey to determine the distribution of these two proteins in a previously under-sampled group, the Euglenozoa. EF-1α was found to be widespread and monophyletic, suggesting it is ancestral in this group. EFL was found in some species belonging to each of the three euglenozoan lineages, diplonemids, kinetoplastids, and euglenids. Conclusions/Significance: Interestingly, the kinetoplastid EFL sequences are specifically related despite the fact that the lineages in which they are found are not sisters to one another, suggesting that EFL and EF-1α co-occurred in an early ancestor of kinetoplastids. This represents the strongest phylogenetic evidence to date that differential loss has contributed to the complex distribution of EFL and EF-1α.

Original languageEnglish (US)
Article numbere5162
JournalPLoS One
Volume4
Issue number4
DOIs
StatePublished - Apr 9 2009
Externally publishedYes

Fingerprint

Euglenozoa
Peptide Elongation Factor 1
Phylogeny
eukaryotic cells
phylogeny
proteins
Eukaryota
ribosomes
translation (genetics)
ancestry
Euglenida
Peptide Elongation Factors
organisms
Proteins
Expressed Sequence Tags
Transfer RNA
Ribosomes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Gile, G., Faktorová, D., Castlejohn, C. A., Burger, G., Lang, B. F., Farmer, M. A., ... Keeling, P. J. (2009). Distribution and phylogeny of EFL and EF-1α in Euglenozoa Suggest ancestral co-occurrence followed by differential loss. PLoS One, 4(4), [e5162]. https://doi.org/10.1371/journal.pone.0005162

Distribution and phylogeny of EFL and EF-1α in Euglenozoa Suggest ancestral co-occurrence followed by differential loss. / Gile, Gillian; Faktorová, Drahomíra; Castlejohn, Christina A.; Burger, Gertraud; Lang, B. Franz; Farmer, Mark A.; Lukeš, Julius; Keeling, Patrick J.

In: PLoS One, Vol. 4, No. 4, e5162, 09.04.2009.

Research output: Contribution to journalArticle

Gile, G, Faktorová, D, Castlejohn, CA, Burger, G, Lang, BF, Farmer, MA, Lukeš, J & Keeling, PJ 2009, 'Distribution and phylogeny of EFL and EF-1α in Euglenozoa Suggest ancestral co-occurrence followed by differential loss', PLoS One, vol. 4, no. 4, e5162. https://doi.org/10.1371/journal.pone.0005162
Gile, Gillian ; Faktorová, Drahomíra ; Castlejohn, Christina A. ; Burger, Gertraud ; Lang, B. Franz ; Farmer, Mark A. ; Lukeš, Julius ; Keeling, Patrick J. / Distribution and phylogeny of EFL and EF-1α in Euglenozoa Suggest ancestral co-occurrence followed by differential loss. In: PLoS One. 2009 ; Vol. 4, No. 4.
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