@inbook{7bef67edc40a411c806f6ba24c5f8caa,
title = "Diolistic labeling and analysis of dendritic spines",
abstract = "Dendritic spines are diverse and plastic components of the neuronal cell apparatus and are highly responsive to trophic factors during both development and adulthood. Diolistic labeling of neurons with lipophilic fluorescent dyes, coupled with advanced high-resolution microscopy methods, provides robust labeling of dendritic spines for assessment of their density and morphology. Here, we describe a method for labeling of dendritic spines using diolistic labeling in ex vivo brain slices, visualization using confocal laser scanning microscopy, deconvolution, and analysis using the Surpass module of Bitplane Imaris software.",
keywords = "3-D reconstruction, Confocal imaging, Deconvolution, Dendritic spine, Diolistic, Lipophilic dye, Morphology",
author = "Michael Olive and {Del Franco}, {Armani P.} and Cassandra Gipson-Reichardt",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC 2018.",
year = "2018",
doi = "10.1007/978-1-4939-7571-6_14",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "179--200",
booktitle = "Methods in Molecular Biology",
}