Diolistic labeling and analysis of dendritic spines

Michael Olive, Armani P. Del Franco, Cassandra Gipson-Reichardt

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

Dendritic spines are diverse and plastic components of the neuronal cell apparatus and are highly responsive to trophic factors during both development and adulthood. Diolistic labeling of neurons with lipophilic fluorescent dyes, coupled with advanced high-resolution microscopy methods, provides robust labeling of dendritic spines for assessment of their density and morphology. Here, we describe a method for labeling of dendritic spines using diolistic labeling in ex vivo brain slices, visualization using confocal laser scanning microscopy, deconvolution, and analysis using the Surpass module of Bitplane Imaris software.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages179-200
Number of pages22
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1727
ISSN (Print)1064-3745

Keywords

  • 3-D reconstruction
  • Confocal imaging
  • Deconvolution
  • Dendritic spine
  • Diolistic
  • Lipophilic dye
  • Morphology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Olive, M., Del Franco, A. P., & Gipson-Reichardt, C. (2018). Diolistic labeling and analysis of dendritic spines. In Methods in Molecular Biology (pp. 179-200). (Methods in Molecular Biology; Vol. 1727). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7571-6_14