Differentiation of Escherichia coli serotypes using DC gradient insulator dielectrophoresis

Paul V. Jones, Alexa F. DeMichele, LaKeta Kemp, Mark Hayes

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Bacteria play a significant role in both human health and disease. An estimated 9.4 million cases of foodborne illness occur in the United States each year. As a result, rapid identification and characterization of microorganisms remains an important research objective. Despite limitations, selective culturing retains a central role among a cadre of identification strategies. For the past decade, separations-based approaches to rapid bacterial identification have been under investigation. Gradient insulator dielectrophoresis (g-iDEP) promises benefits in the form of rapid and specific separation of very similar bacteria, including serotypes of a single species. Furthermore, this approach allows simultaneous concentration of analyte, facilitating detection and downstream analysis. Differentiation of three serotypes or strains of Escherichia coli bacteria is demonstrated within a single g-iDEP microchannel, based on their characteristic electrokinetic properties. Whole cells were captured and concentrated using a range of applied potentials, which generated average electric fields between 160 and 470 V/cm. Bacteria remained viable after exposure to these fields, as determined by cellular motility. These results indicate the potential g-iDEP holds in terms of both separatory power and the possibility for diagnostic applications.

Original languageEnglish (US)
Pages (from-to)183-192
Number of pages10
JournalAnalytical and bioanalytical chemistry
Volume406
Issue number1
DOIs
StatePublished - Jan 2014

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Keywords

  • Bioanalyticalmethods
  • Dielectrophoresis
  • Electrokinetic separations
  • Escherichia coli
  • Microfluidics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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