@article{2b2a471b26834d20940ca649d652b690,
title = "Detection of human, porcine and canine picornaviruses in municipal sewage sludge using pan-enterovirus amplicon-based long-read Illumina sequencing",
abstract = "We describe the successful detection of human, porcine and canine picornaviruses (CanPV) in sewage sludge (at each stage of treatment) from Louisville, Kentucky, USA, using Pan-enterovirus amplicon-based long-read Illumina sequencing. Based on publicly available sequence data in GenBank, this is the first detection of CanPV in the USA and the first detection globally using wastewater-based epidemiology. Our findings also suggest there might be clusters of endemic porcine enterovirus (which have been shown capable of causing systemic infection in porcine) circulation in the USA that have not been sampled for around two decades. Our findings highlight the value of WBE coupled with amplicon based long-read Illumina sequencing for virus surveillance and demonstrates this approach can provide an avenue that supports a “One Health” model to virus surveillance. Finally, we describe a new CanPV assay targeting the capsid protein gene region that can be used globally, especially in resource limited settings for its detection and molecular epidemiology.",
keywords = "high-throughput nucleotide sequencing, Kentucky, One Health, Picornaviridae, wastewater-based epidemiological monitoring",
author = "Faleye, {Temitope O.C.} and Driver, {Erin M.} and Bowes, {Devin A.} and Holm, {Rochelle H.} and Daymond Talley and Ray Yeager and Aruni Bhatnagar and Ted Smith and Arvind Varsani and Halden, {Rolf U.} and Matthew Scotch",
note = "Funding Information: Research reported in this publication was supported by the National Institutes of Health, National Library of Medicine [award number U01LM013129] (to AV, RUH, and MS). Research reported in this publication was also supported by the Chemical, Bioengineering, Environmental and Transport Systems (CBET) Division of the National Science Foundation [award number 2028564]. This work was supported in part by a contract from the Louisville-Jefferson County Metro Government as a component of the Coronavirus Aid, Relief, and Economic Security Act, as well as grants from the James Graham Brown Foundation and the Owsley Brown II Family Foundation. The content is solely the responsibility of the authors and does not necessarily represent the official views of the USA National Institutes of Health or the National Science Foundation. The authors thank the supporting MSD Operations Field Services staff. We also thank the Genomics Core at Arizona State University for help with Sanger sequencing and Loop Genomics for help with Long-Read Illumina sequencing. We especially thank Tuval Ben Yehezkel for post-sequencing support. Publisher Copyright: {\textcopyright} 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd.",
year = "2022",
doi = "10.1080/22221751.2022.2071173",
language = "English (US)",
volume = "11",
pages = "1339--1342",
journal = "Emerging Microbes and Infections",
issn = "2222-1751",
publisher = "Nature Publishing Group",
number = "1",
}
