Detecting Necroptosis in Virus-Infected Cells

Samantha M. Cotsmire, Mateusz Szczerba, Bertram L. Jacobs

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Necroptosis has been implicated as a critical cell death pathway in cancers, Alzheimer’s and other neurodegenerative diseases, and virus-infected cells. Necroptosis occurs when mixed-lineage kinase domain-like protein (MLKL) punctures the cytoplasmic membrane allowing a rapid influx of water leading to a loss of cellular integrity. As its role in human disease becomes apparent, methods identifying necroptosis will need to be further developed and optimized. Here we describe identification of necroptosis through quantifying cell death with pathway inhibitors and using western blots to identify end points of MLKL activation and protein-protein interactions leading to it.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages199-216
Number of pages18
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2225
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell death
  • DSP-cross-linking
  • Dye exclusion
  • Immunoblots
  • Necroptosis
  • Vaccinia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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