Design and use of multi-affinity surfaces in biomolecular interaction analysis-mass spectrometry (BIA/MS): A step toward the design of SPR/MS arrays

Dobrin Nedelkov, Randall W. Nelson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The feasibility of multi-affinity ligand surfaces in biomolecular interaction analysis-mass spectrometry (BIA/MS) was explored in this work. Multi-protein affinity surfaces were constructed by utilizing antibodies to beta-2-microglobulin, cystatin C, retinol binding protein, transthyretin, serum amyloid P and C-reactive protein. In the initial experiments, all six antibodies were immobilized on a single site (flow cell) on the sensor chip surface, followed by verification of the surface activity via separate injections of purified proteins. After an injection of diluted human plasma aliquot over the antibodies-derivatized surfaces, and subsequent MALDI-TOF MS analysis, signals representing five out of the six targeted proteins were observed in the mass spectra. Further, to avoid the complexity of the spectra, the six proteins were divided into two groups (according to their molecular weight) and immobilized on two separate surfaces on a single sensor chip, followed by an injection of human plasma aliquot. The resulting mass spectra showed signals from all proteins. Also, the convolution resulting from the multiply charged ion species was eliminated. The ability to create such multi-affinity surfaces indicates that smaller-size ligand areas/spots can be employed in the BIA/MS protein interaction screening experiments, and opens up the possibilities for construction of novel multi-arrayed SPR-MS platforms and methods for high-throughput parallel protein interaction investigations.

Original languageEnglish (US)
Pages (from-to)15-19
Number of pages5
JournalJournal of Molecular Recognition
Volume16
Issue number1
DOIs
StatePublished - Jan 2003

Fingerprint

Mass spectrometry
Mass Spectrometry
Proteins
Plasma (human)
Antibodies
Injections
Immobilized Antibodies
Ligands
Cystatin C
beta 2-Microglobulin
Retinol-Binding Proteins
Prealbumin
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Amyloid
Signal analysis
Sensors
C-Reactive Protein
Convolution
Membrane Proteins
Molecular Weight

Keywords

  • Array
  • BIA/MS
  • Biosensor
  • Chip
  • Ligands
  • MALDI-TOF mass spectrometry
  • Multi-affinity
  • SPR
  • Surface

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Computer Vision and Pattern Recognition
  • Immunology
  • Molecular Biology

Cite this

Design and use of multi-affinity surfaces in biomolecular interaction analysis-mass spectrometry (BIA/MS) : A step toward the design of SPR/MS arrays. / Nedelkov, Dobrin; Nelson, Randall W.

In: Journal of Molecular Recognition, Vol. 16, No. 1, 01.2003, p. 15-19.

Research output: Contribution to journalArticle

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