Damage-free vibrational spectroscopy of biological materials in the electron microscope

Peter Rez, Toshihiro Aoki, Katia March, Dvir Gur, Ondrej L. Krivanek, Niklas Dellby, Tracy C. Lovejoy, Sharon G. Wolf, Hagai Cohen

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Vibrational spectroscopy in the electron microscope would be transformative in the study of biological samples, provided that radiation damage could be prevented. However, electron beams typically create high-energy excitations that severely accelerate sample degradation. Here this major difficulty is overcome using an 'aloof' electron beam, positioned tens of nanometres away from the sample: high-energy excitations are suppressed, while vibrational modes of energies

Original languageEnglish (US)
Article number10945
JournalNature Communications
Volume7
DOIs
StatePublished - Mar 10 2016

Fingerprint

Vibrational spectroscopy
Excitation energy
Biological materials
Electron beams
Spectrum Analysis
Electron microscopes
electron microscopes
Electrons
damage
Radiation damage
electron beams
spectroscopy
radiation damage
Degradation
excitation
energy
vibration mode
Radiation
degradation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Damage-free vibrational spectroscopy of biological materials in the electron microscope. / Rez, Peter; Aoki, Toshihiro; March, Katia; Gur, Dvir; Krivanek, Ondrej L.; Dellby, Niklas; Lovejoy, Tracy C.; Wolf, Sharon G.; Cohen, Hagai.

In: Nature Communications, Vol. 7, 10945, 10.03.2016.

Research output: Contribution to journalArticle

Rez, P, Aoki, T, March, K, Gur, D, Krivanek, OL, Dellby, N, Lovejoy, TC, Wolf, SG & Cohen, H 2016, 'Damage-free vibrational spectroscopy of biological materials in the electron microscope', Nature Communications, vol. 7, 10945. https://doi.org/10.1038/ncomms10945
Rez, Peter ; Aoki, Toshihiro ; March, Katia ; Gur, Dvir ; Krivanek, Ondrej L. ; Dellby, Niklas ; Lovejoy, Tracy C. ; Wolf, Sharon G. ; Cohen, Hagai. / Damage-free vibrational spectroscopy of biological materials in the electron microscope. In: Nature Communications. 2016 ; Vol. 7.
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