Cross-linked glucose oxidase clusters for biofuel cell anode catalysts

Jonathan Dudzik, Wen Chi Chang, Arunachala Mada Kannan, Slawomir Filipek, Sowmya Viswanathan, Pingzuo Li, V. Renugopalakrishnan, Gerald F. Audette

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The efficient localization of increased levels of active enzymes onto conducting scaffolds is important for the development of enzyme-based biofuel cells. Cross-linked enzyme clusters (CEC) of glucose oxidase (GOx) constrained to functionalized carbon nanotubes (CEC-CNTs) were generated in order to evaluate the potential of using CECs for developing GOx-based bioanodes functioning via direct electron transfer from the GOx active site to the CNT scaffold. CEC-CNTs generated from several weight-to-weight ratios of GOx:CNT were examined for comparable catalytic activity to free GOx into the solution, with CEC-CNTs generated from a 100% GOx solution displaying the greatest enzymatic activity. Scanning transmission electron microscopic analysis of CEC-CNTs generated from 100% GOx to CNT (wt/wt) ratios revealed that CEC clusters of ∼78 μm2 localized to the CNT surface. Electrochemical analysis indicates that the enzyme is engaged in direct electron transfer, and biofuel cells generated using GOx CEC-CNT bioanodes were observed to have a peak power density of ∼180 μW cm-2. These data indicate that the generation of nano-to-micro-sized active enzyme clusters is an attractive option for the design of enzyme-specific biofuel cell powered implantable devices.

Original languageEnglish (US)
Article number035009
JournalBiofabrication
Volume5
Issue number3
DOIs
StatePublished - Sep 2013

Fingerprint

Bioelectric Energy Sources
Biological fuel cells
Glucose Oxidase
Glucose oxidase
Anodes
Electrodes
Enzymes
Catalysts
Electrons
Scaffolds (biology)
Scaffolds
Weights and Measures
Carbon Nanotubes
Catalyst activity
Carbon nanotubes
Catalytic Domain

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering
  • Medicine(all)

Cite this

Dudzik, J., Chang, W. C., Mada Kannan, A., Filipek, S., Viswanathan, S., Li, P., ... Audette, G. F. (2013). Cross-linked glucose oxidase clusters for biofuel cell anode catalysts. Biofabrication, 5(3), [035009]. https://doi.org/10.1088/1758-5082/5/3/035009

Cross-linked glucose oxidase clusters for biofuel cell anode catalysts. / Dudzik, Jonathan; Chang, Wen Chi; Mada Kannan, Arunachala; Filipek, Slawomir; Viswanathan, Sowmya; Li, Pingzuo; Renugopalakrishnan, V.; Audette, Gerald F.

In: Biofabrication, Vol. 5, No. 3, 035009, 09.2013.

Research output: Contribution to journalArticle

Dudzik, J, Chang, WC, Mada Kannan, A, Filipek, S, Viswanathan, S, Li, P, Renugopalakrishnan, V & Audette, GF 2013, 'Cross-linked glucose oxidase clusters for biofuel cell anode catalysts', Biofabrication, vol. 5, no. 3, 035009. https://doi.org/10.1088/1758-5082/5/3/035009
Dudzik, Jonathan ; Chang, Wen Chi ; Mada Kannan, Arunachala ; Filipek, Slawomir ; Viswanathan, Sowmya ; Li, Pingzuo ; Renugopalakrishnan, V. ; Audette, Gerald F. / Cross-linked glucose oxidase clusters for biofuel cell anode catalysts. In: Biofabrication. 2013 ; Vol. 5, No. 3.
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