Abstract
The efficient localization of increased levels of active enzymes onto conducting scaffolds is important for the development of enzyme-based biofuel cells. Cross-linked enzyme clusters (CEC) of glucose oxidase (GOx) constrained to functionalized carbon nanotubes (CEC-CNTs) were generated in order to evaluate the potential of using CECs for developing GOx-based bioanodes functioning via direct electron transfer from the GOx active site to the CNT scaffold. CEC-CNTs generated from several weight-to-weight ratios of GOx:CNT were examined for comparable catalytic activity to free GOx into the solution, with CEC-CNTs generated from a 100% GOx solution displaying the greatest enzymatic activity. Scanning transmission electron microscopic analysis of CEC-CNTs generated from 100% GOx to CNT (wt/wt) ratios revealed that CEC clusters of ∼78 μm2 localized to the CNT surface. Electrochemical analysis indicates that the enzyme is engaged in direct electron transfer, and biofuel cells generated using GOx CEC-CNT bioanodes were observed to have a peak power density of ∼180 μW cm-2. These data indicate that the generation of nano-to-micro-sized active enzyme clusters is an attractive option for the design of enzyme-specific biofuel cell powered implantable devices.
| Original language | English (US) |
|---|---|
| Article number | 035009 |
| Journal | Biofabrication |
| Volume | 5 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 2013 |
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ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Biomaterials
- Bioengineering
- Biomedical Engineering
- Medicine(all)
Cite this
Cross-linked glucose oxidase clusters for biofuel cell anode catalysts. / Dudzik, Jonathan; Chang, Wen Chi; Mada Kannan, Arunachala; Filipek, Slawomir; Viswanathan, Sowmya; Li, Pingzuo; Renugopalakrishnan, V.; Audette, Gerald F.
In: Biofabrication, Vol. 5, No. 3, 035009, 09.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cross-linked glucose oxidase clusters for biofuel cell anode catalysts
AU - Dudzik, Jonathan
AU - Chang, Wen Chi
AU - Mada Kannan, Arunachala
AU - Filipek, Slawomir
AU - Viswanathan, Sowmya
AU - Li, Pingzuo
AU - Renugopalakrishnan, V.
AU - Audette, Gerald F.
PY - 2013/9
Y1 - 2013/9
N2 - The efficient localization of increased levels of active enzymes onto conducting scaffolds is important for the development of enzyme-based biofuel cells. Cross-linked enzyme clusters (CEC) of glucose oxidase (GOx) constrained to functionalized carbon nanotubes (CEC-CNTs) were generated in order to evaluate the potential of using CECs for developing GOx-based bioanodes functioning via direct electron transfer from the GOx active site to the CNT scaffold. CEC-CNTs generated from several weight-to-weight ratios of GOx:CNT were examined for comparable catalytic activity to free GOx into the solution, with CEC-CNTs generated from a 100% GOx solution displaying the greatest enzymatic activity. Scanning transmission electron microscopic analysis of CEC-CNTs generated from 100% GOx to CNT (wt/wt) ratios revealed that CEC clusters of ∼78 μm2 localized to the CNT surface. Electrochemical analysis indicates that the enzyme is engaged in direct electron transfer, and biofuel cells generated using GOx CEC-CNT bioanodes were observed to have a peak power density of ∼180 μW cm-2. These data indicate that the generation of nano-to-micro-sized active enzyme clusters is an attractive option for the design of enzyme-specific biofuel cell powered implantable devices.
AB - The efficient localization of increased levels of active enzymes onto conducting scaffolds is important for the development of enzyme-based biofuel cells. Cross-linked enzyme clusters (CEC) of glucose oxidase (GOx) constrained to functionalized carbon nanotubes (CEC-CNTs) were generated in order to evaluate the potential of using CECs for developing GOx-based bioanodes functioning via direct electron transfer from the GOx active site to the CNT scaffold. CEC-CNTs generated from several weight-to-weight ratios of GOx:CNT were examined for comparable catalytic activity to free GOx into the solution, with CEC-CNTs generated from a 100% GOx solution displaying the greatest enzymatic activity. Scanning transmission electron microscopic analysis of CEC-CNTs generated from 100% GOx to CNT (wt/wt) ratios revealed that CEC clusters of ∼78 μm2 localized to the CNT surface. Electrochemical analysis indicates that the enzyme is engaged in direct electron transfer, and biofuel cells generated using GOx CEC-CNT bioanodes were observed to have a peak power density of ∼180 μW cm-2. These data indicate that the generation of nano-to-micro-sized active enzyme clusters is an attractive option for the design of enzyme-specific biofuel cell powered implantable devices.
UR - http://www.scopus.com/inward/record.url?scp=84883126884&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883126884&partnerID=8YFLogxK
U2 - 10.1088/1758-5082/5/3/035009
DO - 10.1088/1758-5082/5/3/035009
M3 - Article
C2 - 23880606
AN - SCOPUS:84883126884
VL - 5
JO - Biofabrication
JF - Biofabrication
SN - 1758-5082
IS - 3
M1 - 035009
ER -