Controlling ERK Activation Dynamics in Mammary Epithelial Cells with Alternating Electric Fields through Microelectrodes

Liang Guo, Houpu Li, Yuan Wang, Zhuo Li, John Albeck, Min Zhao, Quan Qing

Research output: Contribution to journalArticle

Abstract

Amplitude, duration, and frequency of activation of the extracellular-signal-regulated kinase (ERK) pathway code distinct information to instruct cells to migrate, proliferate, or differentiate. Synchronized frequency control of ERK activation would provide a powerful approach to regulate cell behaviors. Here we demonstrated modulation of ERK activities using alternative current (AC) electric fields (EFs) applied through high-k dielectric passivated microelectrodes. Both the amplitude and frequency of ERK activation can be precisely synchronized and modulated. ERK activation in our system is independent of Faradaic currents and electroporation, thus excluding mechanisms of changes in pH, reactive oxygen species, and other electrochemical reaction. Further experiments pinpointed a mechanism of phosphorylation site of epidermal growth factor (EGF) receptor to activate the EGFR-ERK pathway, and independent of EGF. AC EFs thus provide a powerful platform for practical and precise control of EGFR-ERK pathway.

Original languageEnglish (US)
Pages (from-to)7526-7533
Number of pages8
JournalNano Letters
Volume19
Issue number10
DOIs
StatePublished - Oct 9 2019

Fingerprint

Microelectrodes
Extracellular Signal-Regulated MAP Kinases
Chemical activation
Electric fields
activation
electric fields
Phosphorylation
frequency control
Modulation
phosphorylation
Oxygen
Epithelial Cells
cells
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Reactive Oxygen Species
platforms
Experiments
modulation
oxygen

Keywords

  • EGFR
  • electric field stimulation
  • ERK pathway
  • microelectrode

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Controlling ERK Activation Dynamics in Mammary Epithelial Cells with Alternating Electric Fields through Microelectrodes. / Guo, Liang; Li, Houpu; Wang, Yuan; Li, Zhuo; Albeck, John; Zhao, Min; Qing, Quan.

In: Nano Letters, Vol. 19, No. 10, 09.10.2019, p. 7526-7533.

Research output: Contribution to journalArticle

Guo, Liang ; Li, Houpu ; Wang, Yuan ; Li, Zhuo ; Albeck, John ; Zhao, Min ; Qing, Quan. / Controlling ERK Activation Dynamics in Mammary Epithelial Cells with Alternating Electric Fields through Microelectrodes. In: Nano Letters. 2019 ; Vol. 19, No. 10. pp. 7526-7533.
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