Competitive release and facilitation of drug-resistant parasites after therapeutic chemotherapy in a rodent malaria model

Andrew R. Wargo, Silvie Huijben, Jacobus C. De Roode, James Shepherd, Andrew F. Read

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Malaria infections frequently consist of mixtures of drug-resistant and drug-sensitive parasites. If crowding occurs, where clonal population densities are suppressed by the presence of coinfecting clones, removal of susceptible clones by drug treatment could allow resistant clones to expand into the newly vacated niche space within a host. Theoretical models show that, if such competitive release occurs, it can be a potent contributor to the strength of selection, greatly accelerating the rate at which resistance spreads in a population. A variety of correlational field data suggest that competitive release could occur in human malaria populations, but direct evidence cannot be ethically obtained from human infections. Here we show competitive release after pyrimethamine curative chemotherapy of acute infections of the rodent malaria Plasmodium chabaudi in laboratory mice. The expansion of resistant parasite numbers after treatment resulted in enhanced transmission-stage densities. After the elimination or near-elimination of sensitive parasites, the number of resistant parasites increased beyond that achieved when a competitor had never been present. Thus, a substantial competitive release occurred, markedly elevating the fitness advantages of drug resistance above those arising from survival alone. This finding may explain the rapid spread of drug resistance and the subsequently brief useful lifespans of some antimalarial drugs. In a second experiment, where subcurative chemotherapy was administered, the resistant clone was only partly released from competitive suppression and experienced a restriction in the size of its expansion after treatment. This finding raises the prospect of harnessing in-host ecology to slow the spread of drug resistance.

Original languageEnglish (US)
Pages (from-to)19914-19919
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number50
DOIs
StatePublished - Dec 11 2007
Externally publishedYes

Fingerprint

Malaria
Rodentia
Parasites
Clone Cells
Drug Resistance
Drug Therapy
Infection
Plasmodium chabaudi
Pharmaceutical Preparations
Pyrimethamine
Crowding
Antimalarials
Therapeutics
Population Density
Ecology
Population
Theoretical Models
Survival
Drug Liberation

Keywords

  • Competition
  • Evolution of drug resistance
  • In-host ecology
  • Plasmodium chabaudi
  • Transmission

ASJC Scopus subject areas

  • General

Cite this

Competitive release and facilitation of drug-resistant parasites after therapeutic chemotherapy in a rodent malaria model. / Wargo, Andrew R.; Huijben, Silvie; De Roode, Jacobus C.; Shepherd, James; Read, Andrew F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 50, 11.12.2007, p. 19914-19919.

Research output: Contribution to journalArticle

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