Combined TIRF-AFM setup: Controlled quenching of individual quantum dots

Rainer Eckel, Volker Walhorn, Christoph Pelargus, Jörg Martini, Thomas Nann, Dario Anselmetti, Robert Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Single molecules can nowadays be investigated by means of optical, mechanical and electrical methods. Fluorescence imaging and spectroscopy yield valuable and quantitative information about the optical properties and the spatial distribution of single molecules. Force spectroscopy by atomic force microscopy (AFM) or optical tweezers allows addressing, manipulation and quantitative probing of the nanomechanical properties of individual macromolecules. We present a combined AFM and total internal reflection fluorescence (TIRF) microscopy setup that enables ultrasensitive laser induced fluorescence detection of individual fluorophores, control of the AFM probe position in x, y and z-direction with nanometer precision, and simultaneous investigation of optical and mechanical properties at the single molecule level. Here, we present the distance-controlled quenching of semiconductor quantum dot clusters with an AFM tip. In future applications, fluorescence resonant energy transfer between single donor and acceptor molecules will be investigated.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6092
DOIs
StatePublished - 2006
Externally publishedYes
EventUltrasensitive and Single-Molecule Detection Technologies - San Jose, CA, United States
Duration: Jan 21 2006Jan 24 2006

Other

OtherUltrasensitive and Single-Molecule Detection Technologies
CountryUnited States
CitySan Jose, CA
Period1/21/061/24/06

Fingerprint

Fluorescence microscopy
Semiconductor quantum dots
Quenching
Atomic force microscopy
Molecules
Fluorescence
Optical properties
Spectroscopy
Optical tweezers
Fluorophores
Macromolecules
Energy transfer
Spatial distribution
Imaging techniques
Mechanical properties
Lasers

Keywords

  • AFM
  • Quantum Dots
  • Single Molecule Manipulation
  • TIRF

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Eckel, R., Walhorn, V., Pelargus, C., Martini, J., Nann, T., Anselmetti, D., & Ros, R. (2006). Combined TIRF-AFM setup: Controlled quenching of individual quantum dots. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6092). [609209] https://doi.org/10.1117/12.645067

Combined TIRF-AFM setup : Controlled quenching of individual quantum dots. / Eckel, Rainer; Walhorn, Volker; Pelargus, Christoph; Martini, Jörg; Nann, Thomas; Anselmetti, Dario; Ros, Robert.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6092 2006. 609209.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Eckel, R, Walhorn, V, Pelargus, C, Martini, J, Nann, T, Anselmetti, D & Ros, R 2006, Combined TIRF-AFM setup: Controlled quenching of individual quantum dots. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6092, 609209, Ultrasensitive and Single-Molecule Detection Technologies, San Jose, CA, United States, 1/21/06. https://doi.org/10.1117/12.645067
Eckel R, Walhorn V, Pelargus C, Martini J, Nann T, Anselmetti D et al. Combined TIRF-AFM setup: Controlled quenching of individual quantum dots. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6092. 2006. 609209 https://doi.org/10.1117/12.645067
Eckel, Rainer ; Walhorn, Volker ; Pelargus, Christoph ; Martini, Jörg ; Nann, Thomas ; Anselmetti, Dario ; Ros, Robert. / Combined TIRF-AFM setup : Controlled quenching of individual quantum dots. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6092 2006.
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