Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source

Hemanth K.N. Reddy, Chun Hong Yoon, Andrew Aquila, Salah Awel, Kartik Ayyer, Anton Barty, Peter Berntsen, Johan Bielecki, Sergey Bobkov, Maximilian Bucher, Gabriella A. Carini, Sebastian Carron, Henry Chapman, Benedikt Daurer, Hasan Demirci, Tomas Ekeberg, Petra Fromme, Janos Hajdu, Max Felix Hanke, Philip Hart & 25 others Brenda Hogue, Ahmad Hosseinizadeh, Yoonhee Kim, Richard Kirian, Ruslan P. Kurta, Daniel S.D. Larsson, N. Duane Loh, Filipe R.N.C. Maia, Adrian P. Mancuso, Kerstin Mühlig, Anna Munke, Daewoong Nam, Carl Nettelblad, Abbas Ourmazd, Max Rose, Peter Schwander, Marvin Seibert, Jonas A. Sellberg, Changyong Song, John Spence, Martin Svenda, Gijs Van Der Schot, Ivan A. Vartanyants, Garth J. Williams, P. Lourdu Xavier

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported ∼600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.

Original languageEnglish (US)
Article number170079
JournalScientific data
Volume4
DOIs
StatePublished - Jun 27 2017

Fingerprint

X ray lasers
Molecular imaging
Soft X-ray
Free electron lasers
Viruses
X-ray Diffraction
Molecular structure
statistical analysis
Light sources
Optics
Statistical methods
reconstruction
Diffraction
Crystallization
Imaging
regime
Detectors
Imaging techniques
X ray diffraction
experiment

ASJC Scopus subject areas

  • Education
  • Library and Information Sciences
  • Computer Science Applications
  • Information Systems
  • Statistics, Probability and Uncertainty
  • Statistics and Probability

Cite this

Reddy, H. K. N., Yoon, C. H., Aquila, A., Awel, S., Ayyer, K., Barty, A., ... Lourdu Xavier, P. (2017). Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source. Scientific data, 4, [170079]. https://doi.org/10.1038/sdata.2017.79

Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source. / Reddy, Hemanth K.N.; Yoon, Chun Hong; Aquila, Andrew; Awel, Salah; Ayyer, Kartik; Barty, Anton; Berntsen, Peter; Bielecki, Johan; Bobkov, Sergey; Bucher, Maximilian; Carini, Gabriella A.; Carron, Sebastian; Chapman, Henry; Daurer, Benedikt; Demirci, Hasan; Ekeberg, Tomas; Fromme, Petra; Hajdu, Janos; Hanke, Max Felix; Hart, Philip; Hogue, Brenda; Hosseinizadeh, Ahmad; Kim, Yoonhee; Kirian, Richard; Kurta, Ruslan P.; Larsson, Daniel S.D.; Duane Loh, N.; Maia, Filipe R.N.C.; Mancuso, Adrian P.; Mühlig, Kerstin; Munke, Anna; Nam, Daewoong; Nettelblad, Carl; Ourmazd, Abbas; Rose, Max; Schwander, Peter; Seibert, Marvin; Sellberg, Jonas A.; Song, Changyong; Spence, John; Svenda, Martin; Van Der Schot, Gijs; Vartanyants, Ivan A.; Williams, Garth J.; Lourdu Xavier, P.

In: Scientific data, Vol. 4, 170079, 27.06.2017.

Research output: Contribution to journalArticle

Reddy, HKN, Yoon, CH, Aquila, A, Awel, S, Ayyer, K, Barty, A, Berntsen, P, Bielecki, J, Bobkov, S, Bucher, M, Carini, GA, Carron, S, Chapman, H, Daurer, B, Demirci, H, Ekeberg, T, Fromme, P, Hajdu, J, Hanke, MF, Hart, P, Hogue, B, Hosseinizadeh, A, Kim, Y, Kirian, R, Kurta, RP, Larsson, DSD, Duane Loh, N, Maia, FRNC, Mancuso, AP, Mühlig, K, Munke, A, Nam, D, Nettelblad, C, Ourmazd, A, Rose, M, Schwander, P, Seibert, M, Sellberg, JA, Song, C, Spence, J, Svenda, M, Van Der Schot, G, Vartanyants, IA, Williams, GJ & Lourdu Xavier, P 2017, 'Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source', Scientific data, vol. 4, 170079. https://doi.org/10.1038/sdata.2017.79
Reddy, Hemanth K.N. ; Yoon, Chun Hong ; Aquila, Andrew ; Awel, Salah ; Ayyer, Kartik ; Barty, Anton ; Berntsen, Peter ; Bielecki, Johan ; Bobkov, Sergey ; Bucher, Maximilian ; Carini, Gabriella A. ; Carron, Sebastian ; Chapman, Henry ; Daurer, Benedikt ; Demirci, Hasan ; Ekeberg, Tomas ; Fromme, Petra ; Hajdu, Janos ; Hanke, Max Felix ; Hart, Philip ; Hogue, Brenda ; Hosseinizadeh, Ahmad ; Kim, Yoonhee ; Kirian, Richard ; Kurta, Ruslan P. ; Larsson, Daniel S.D. ; Duane Loh, N. ; Maia, Filipe R.N.C. ; Mancuso, Adrian P. ; Mühlig, Kerstin ; Munke, Anna ; Nam, Daewoong ; Nettelblad, Carl ; Ourmazd, Abbas ; Rose, Max ; Schwander, Peter ; Seibert, Marvin ; Sellberg, Jonas A. ; Song, Changyong ; Spence, John ; Svenda, Martin ; Van Der Schot, Gijs ; Vartanyants, Ivan A. ; Williams, Garth J. ; Lourdu Xavier, P. / Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source. In: Scientific data. 2017 ; Vol. 4.
@article{f020b2e0caea497a9ee523ff1a4d41d8,
title = "Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source",
abstract = "Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported ∼600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.",
author = "Reddy, {Hemanth K.N.} and Yoon, {Chun Hong} and Andrew Aquila and Salah Awel and Kartik Ayyer and Anton Barty and Peter Berntsen and Johan Bielecki and Sergey Bobkov and Maximilian Bucher and Carini, {Gabriella A.} and Sebastian Carron and Henry Chapman and Benedikt Daurer and Hasan Demirci and Tomas Ekeberg and Petra Fromme and Janos Hajdu and Hanke, {Max Felix} and Philip Hart and Brenda Hogue and Ahmad Hosseinizadeh and Yoonhee Kim and Richard Kirian and Kurta, {Ruslan P.} and Larsson, {Daniel S.D.} and {Duane Loh}, N. and Maia, {Filipe R.N.C.} and Mancuso, {Adrian P.} and Kerstin M{\"u}hlig and Anna Munke and Daewoong Nam and Carl Nettelblad and Abbas Ourmazd and Max Rose and Peter Schwander and Marvin Seibert and Sellberg, {Jonas A.} and Changyong Song and John Spence and Martin Svenda and {Van Der Schot}, Gijs and Vartanyants, {Ivan A.} and Williams, {Garth J.} and {Lourdu Xavier}, P.",
year = "2017",
month = "6",
day = "27",
doi = "10.1038/sdata.2017.79",
language = "English (US)",
volume = "4",
journal = "Scientific data",
issn = "2052-4463",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source

AU - Reddy, Hemanth K.N.

AU - Yoon, Chun Hong

AU - Aquila, Andrew

AU - Awel, Salah

AU - Ayyer, Kartik

AU - Barty, Anton

AU - Berntsen, Peter

AU - Bielecki, Johan

AU - Bobkov, Sergey

AU - Bucher, Maximilian

AU - Carini, Gabriella A.

AU - Carron, Sebastian

AU - Chapman, Henry

AU - Daurer, Benedikt

AU - Demirci, Hasan

AU - Ekeberg, Tomas

AU - Fromme, Petra

AU - Hajdu, Janos

AU - Hanke, Max Felix

AU - Hart, Philip

AU - Hogue, Brenda

AU - Hosseinizadeh, Ahmad

AU - Kim, Yoonhee

AU - Kirian, Richard

AU - Kurta, Ruslan P.

AU - Larsson, Daniel S.D.

AU - Duane Loh, N.

AU - Maia, Filipe R.N.C.

AU - Mancuso, Adrian P.

AU - Mühlig, Kerstin

AU - Munke, Anna

AU - Nam, Daewoong

AU - Nettelblad, Carl

AU - Ourmazd, Abbas

AU - Rose, Max

AU - Schwander, Peter

AU - Seibert, Marvin

AU - Sellberg, Jonas A.

AU - Song, Changyong

AU - Spence, John

AU - Svenda, Martin

AU - Van Der Schot, Gijs

AU - Vartanyants, Ivan A.

AU - Williams, Garth J.

AU - Lourdu Xavier, P.

PY - 2017/6/27

Y1 - 2017/6/27

N2 - Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported ∼600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.

AB - Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported ∼600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.

UR - http://www.scopus.com/inward/record.url?scp=85021625011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021625011&partnerID=8YFLogxK

U2 - 10.1038/sdata.2017.79

DO - 10.1038/sdata.2017.79

M3 - Article

VL - 4

JO - Scientific data

JF - Scientific data

SN - 2052-4463

M1 - 170079

ER -