Co-overexpression of AVP1 and AtNHX1 in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants

Guoxin Shen, Jia Wei, Xiaoyun Qiu, Rongbin Hu, Sundaram Kuppu, Dick Auld, Eduardo Blumwald, Roberto Gaxiola, Paxton Payton, Hong Zhang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Salinity and drought are two major environmental stresses that limit the growth and productivity of cotton. To improve cotton's drought and salt tolerance, transgenic cotton plants expressing the Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1 and H+-pyrophosphatase gene AVP1 were produced by cross-pollination of two single-gene-overexpressing plants. The salt tolerance and drought tolerance were further enhanced by simultaneously overexpressing AVP1 and AtNHX1 in comparison to AVP1 or AtNHX1 single-gene-overexpressing plants and to wild-type plants. Plant height, boll number, and fiber yield of AVP1/AtNHX1-co-overexpressing plants were higher than those of AVP1-overexpressing, AtNHX1-overexpressing, segregated non-transgenic line, and wild-type plants under saline and drought conditions. The photosynthetic rate of AVP1/AtNHX1-co-overexpressing plants was significantly higher than that of single-gene-overexpressing and wild-type plants under 200 mM NaCl treatment. In addition, the root systems of AVP1/AtNHX1-co-overexpressing plants were larger than those of single-gene-overexpressing and wild-type plants, which was likely due to increased auxin polar transport in the root systems of the AVP1/AtNHX1-co-overexpressing plants. Moreover, these AVP1/AtNHX1-co-overexpressing cotton plants produced 24 % higher fiber yield under low-irrigation conditions and 35 % higher fiber yield under dryland conditions as compared to wild-type cotton in the field.

Original languageEnglish (US)
JournalPlant Molecular Biology Reporter
DOIs
StateAccepted/In press - May 28 2014

Fingerprint

Salt-Tolerance
Gossypium
Genetically Modified Plants
Droughts
salt tolerance
drought tolerance
cotton
genetically modified organisms
Plant Genes
Genes
genes
Inorganic Pyrophosphatase
Pollination
Sodium-Hydrogen Antiporter
root systems
Indoleacetic Acids
Salinity
drought
Arabidopsis
pyrophosphatases

Keywords

  • AtNHX1
  • Auxin transport
  • AVP1
  • Cotton
  • Drought tolerance
  • Salt tolerance

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

Cite this

Co-overexpression of AVP1 and AtNHX1 in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants. / Shen, Guoxin; Wei, Jia; Qiu, Xiaoyun; Hu, Rongbin; Kuppu, Sundaram; Auld, Dick; Blumwald, Eduardo; Gaxiola, Roberto; Payton, Paxton; Zhang, Hong.

In: Plant Molecular Biology Reporter, 28.05.2014.

Research output: Contribution to journalArticle

Shen, Guoxin ; Wei, Jia ; Qiu, Xiaoyun ; Hu, Rongbin ; Kuppu, Sundaram ; Auld, Dick ; Blumwald, Eduardo ; Gaxiola, Roberto ; Payton, Paxton ; Zhang, Hong. / Co-overexpression of AVP1 and AtNHX1 in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants. In: Plant Molecular Biology Reporter. 2014.
@article{1d173dddb31a4c70b7a12404a876b3d5,
title = "Co-overexpression of AVP1 and AtNHX1 in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants",
abstract = "Salinity and drought are two major environmental stresses that limit the growth and productivity of cotton. To improve cotton's drought and salt tolerance, transgenic cotton plants expressing the Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1 and H+-pyrophosphatase gene AVP1 were produced by cross-pollination of two single-gene-overexpressing plants. The salt tolerance and drought tolerance were further enhanced by simultaneously overexpressing AVP1 and AtNHX1 in comparison to AVP1 or AtNHX1 single-gene-overexpressing plants and to wild-type plants. Plant height, boll number, and fiber yield of AVP1/AtNHX1-co-overexpressing plants were higher than those of AVP1-overexpressing, AtNHX1-overexpressing, segregated non-transgenic line, and wild-type plants under saline and drought conditions. The photosynthetic rate of AVP1/AtNHX1-co-overexpressing plants was significantly higher than that of single-gene-overexpressing and wild-type plants under 200 mM NaCl treatment. In addition, the root systems of AVP1/AtNHX1-co-overexpressing plants were larger than those of single-gene-overexpressing and wild-type plants, which was likely due to increased auxin polar transport in the root systems of the AVP1/AtNHX1-co-overexpressing plants. Moreover, these AVP1/AtNHX1-co-overexpressing cotton plants produced 24 {\%} higher fiber yield under low-irrigation conditions and 35 {\%} higher fiber yield under dryland conditions as compared to wild-type cotton in the field.",
keywords = "AtNHX1, Auxin transport, AVP1, Cotton, Drought tolerance, Salt tolerance",
author = "Guoxin Shen and Jia Wei and Xiaoyun Qiu and Rongbin Hu and Sundaram Kuppu and Dick Auld and Eduardo Blumwald and Roberto Gaxiola and Paxton Payton and Hong Zhang",
year = "2014",
month = "5",
day = "28",
doi = "10.1007/s11105-014-0739-8",
language = "English (US)",
journal = "Plant Molecular Biology Reporter",
issn = "0735-9640",
publisher = "Springer New York",

}

TY - JOUR

T1 - Co-overexpression of AVP1 and AtNHX1 in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants

AU - Shen, Guoxin

AU - Wei, Jia

AU - Qiu, Xiaoyun

AU - Hu, Rongbin

AU - Kuppu, Sundaram

AU - Auld, Dick

AU - Blumwald, Eduardo

AU - Gaxiola, Roberto

AU - Payton, Paxton

AU - Zhang, Hong

PY - 2014/5/28

Y1 - 2014/5/28

N2 - Salinity and drought are two major environmental stresses that limit the growth and productivity of cotton. To improve cotton's drought and salt tolerance, transgenic cotton plants expressing the Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1 and H+-pyrophosphatase gene AVP1 were produced by cross-pollination of two single-gene-overexpressing plants. The salt tolerance and drought tolerance were further enhanced by simultaneously overexpressing AVP1 and AtNHX1 in comparison to AVP1 or AtNHX1 single-gene-overexpressing plants and to wild-type plants. Plant height, boll number, and fiber yield of AVP1/AtNHX1-co-overexpressing plants were higher than those of AVP1-overexpressing, AtNHX1-overexpressing, segregated non-transgenic line, and wild-type plants under saline and drought conditions. The photosynthetic rate of AVP1/AtNHX1-co-overexpressing plants was significantly higher than that of single-gene-overexpressing and wild-type plants under 200 mM NaCl treatment. In addition, the root systems of AVP1/AtNHX1-co-overexpressing plants were larger than those of single-gene-overexpressing and wild-type plants, which was likely due to increased auxin polar transport in the root systems of the AVP1/AtNHX1-co-overexpressing plants. Moreover, these AVP1/AtNHX1-co-overexpressing cotton plants produced 24 % higher fiber yield under low-irrigation conditions and 35 % higher fiber yield under dryland conditions as compared to wild-type cotton in the field.

AB - Salinity and drought are two major environmental stresses that limit the growth and productivity of cotton. To improve cotton's drought and salt tolerance, transgenic cotton plants expressing the Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1 and H+-pyrophosphatase gene AVP1 were produced by cross-pollination of two single-gene-overexpressing plants. The salt tolerance and drought tolerance were further enhanced by simultaneously overexpressing AVP1 and AtNHX1 in comparison to AVP1 or AtNHX1 single-gene-overexpressing plants and to wild-type plants. Plant height, boll number, and fiber yield of AVP1/AtNHX1-co-overexpressing plants were higher than those of AVP1-overexpressing, AtNHX1-overexpressing, segregated non-transgenic line, and wild-type plants under saline and drought conditions. The photosynthetic rate of AVP1/AtNHX1-co-overexpressing plants was significantly higher than that of single-gene-overexpressing and wild-type plants under 200 mM NaCl treatment. In addition, the root systems of AVP1/AtNHX1-co-overexpressing plants were larger than those of single-gene-overexpressing and wild-type plants, which was likely due to increased auxin polar transport in the root systems of the AVP1/AtNHX1-co-overexpressing plants. Moreover, these AVP1/AtNHX1-co-overexpressing cotton plants produced 24 % higher fiber yield under low-irrigation conditions and 35 % higher fiber yield under dryland conditions as compared to wild-type cotton in the field.

KW - AtNHX1

KW - Auxin transport

KW - AVP1

KW - Cotton

KW - Drought tolerance

KW - Salt tolerance

UR - http://www.scopus.com/inward/record.url?scp=84901584489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901584489&partnerID=8YFLogxK

U2 - 10.1007/s11105-014-0739-8

DO - 10.1007/s11105-014-0739-8

M3 - Article

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

SN - 0735-9640

ER -