Cloning, sequencing, and expression of the mig gene of Mycobacterium avium, which codes for a secreted macrophage-induced protein

Georg Plum, Martina Brenden, Josephine E. Clark-Curtiss, Gerhard Pulverer

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Mycobacterium avium is an intracellular pathogen that has evolved to be a frequent cause of disseminated infection in immunocompromised patients. Although these bacilli are readily phagocytized, they are able to survive and even multiply within human macrophages. The process whereby mycobacteria circumvent the lytic functions of the macrophages is currently not well understood, but this is a key aspect in the pathogenicity of all pathogenic mycobacteria. Previously, we identified a gene in M. avium, designated mig (for macrophage-induced gene), the expression of which is induced when the bacilli grow in human macrophages (G. Plum and J. E. Clark-Curtiss, Infect. Immun. 62:476-483, 1994). In the present study we show that (i) the nucleotide sequence of the mig gene has an open reading frame of 295 amino acids with a strong bias for mycobacterial codon usage, (ii) the mig gene also codes for a putative signal peptide of 19 amino acid residues, (iii) mig is induced by acidity to be expressed as an early-secreted 30-kDa protein, and (iv) the Mig protein exhibits an AMP-binding domain signature. However, beyond this motif which is common to enzymes that activate a large variety of substrates, no homologics to known sequences are found. We also show that (v) Mycobacterium smegmatis strains expressing the Mig protein have a limited advantage for survival in macrophages. These findings may be concordant with a role of the mig gene in the virulence of M. avium.

Original languageEnglish (US)
Pages (from-to)4548-4557
Number of pages10
JournalInfection and Immunity
Volume65
Issue number11
StatePublished - 1997
Externally publishedYes

Fingerprint

Mycobacterium avium
Organism Cloning
Macrophages
Genes
Proteins
Mycobacterium
Bacillus
Virulence
Mycobacterium smegmatis
Amino Acids
Immunocompromised Host
Adenosine Monophosphate
Protein Sorting Signals
Codon
Open Reading Frames
Gene Expression
Survival

ASJC Scopus subject areas

  • Immunology

Cite this

Cloning, sequencing, and expression of the mig gene of Mycobacterium avium, which codes for a secreted macrophage-induced protein. / Plum, Georg; Brenden, Martina; Clark-Curtiss, Josephine E.; Pulverer, Gerhard.

In: Infection and Immunity, Vol. 65, No. 11, 1997, p. 4548-4557.

Research output: Contribution to journalArticle

Plum, Georg ; Brenden, Martina ; Clark-Curtiss, Josephine E. ; Pulverer, Gerhard. / Cloning, sequencing, and expression of the mig gene of Mycobacterium avium, which codes for a secreted macrophage-induced protein. In: Infection and Immunity. 1997 ; Vol. 65, No. 11. pp. 4548-4557.
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