Cloning of the psbK gene from synechocystis sp. PCC 6803 and characterization of photosystem II in mutants lacking PSII-K

Masahiko Ikeuchi, Beth Eggers, Gaozhong Shen, Andrew Webber, Jiujiang Yu, Atsushi Hirano, Yorinao Inoue, Willem Vermaas

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Abstract

We cloned and sequenced the psbK gene, coding for a small photosystem II component (PSII-K), from the transformable cyanobacterium, Synechocystis sp. PCC 6803, and determined the N-terminal sequence of mature PSII-K. The psbK gene product is processed by cleaving off eight amino acid residues from the N terminus. A mutant lacking psbK was constructed; this mutant grew photoautotrophically, but its growth rate was reduced. The number of photosystem II reaction centers on a chlorophyll basis was decreased by less than a factor of 2 in the psbK-deletion mutant. In Synechocystis sp. PCC 6803, the psbK gene is transcribed as a single gene and is not part of an operon. Single-site mutations were introduced into psbK leading to early termination or deletion of the presequence. The phenotype of these mutants strongly resembles that of the psbK deletion mutant, indicating that indeed the change in phenotype in the deletion mutant is directly correlated with PSII-K. PSII-K is not essential for photosystem II assembly or activity but is needed for optimal photosystem II function.

Original languageEnglish (US)
Pages (from-to)11111-11115
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number17
StatePublished - 1991

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Synechocystis
Photosystem II Protein Complex
Cloning
Organism Cloning
Genes
Phenotype
Cyanobacteria
Chlorophyll
Operon
Amino Acids
Mutation
Growth

ASJC Scopus subject areas

  • Biochemistry

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Cloning of the psbK gene from synechocystis sp. PCC 6803 and characterization of photosystem II in mutants lacking PSII-K. / Ikeuchi, Masahiko; Eggers, Beth; Shen, Gaozhong; Webber, Andrew; Yu, Jiujiang; Hirano, Atsushi; Inoue, Yorinao; Vermaas, Willem.

In: Journal of Biological Chemistry, Vol. 266, No. 17, 1991, p. 11111-11115.

Research output: Contribution to journalArticle

Ikeuchi, Masahiko ; Eggers, Beth ; Shen, Gaozhong ; Webber, Andrew ; Yu, Jiujiang ; Hirano, Atsushi ; Inoue, Yorinao ; Vermaas, Willem. / Cloning of the psbK gene from synechocystis sp. PCC 6803 and characterization of photosystem II in mutants lacking PSII-K. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 17. pp. 11111-11115.
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abstract = "We cloned and sequenced the psbK gene, coding for a small photosystem II component (PSII-K), from the transformable cyanobacterium, Synechocystis sp. PCC 6803, and determined the N-terminal sequence of mature PSII-K. The psbK gene product is processed by cleaving off eight amino acid residues from the N terminus. A mutant lacking psbK was constructed; this mutant grew photoautotrophically, but its growth rate was reduced. The number of photosystem II reaction centers on a chlorophyll basis was decreased by less than a factor of 2 in the psbK-deletion mutant. In Synechocystis sp. PCC 6803, the psbK gene is transcribed as a single gene and is not part of an operon. Single-site mutations were introduced into psbK leading to early termination or deletion of the presequence. The phenotype of these mutants strongly resembles that of the psbK deletion mutant, indicating that indeed the change in phenotype in the deletion mutant is directly correlated with PSII-K. PSII-K is not essential for photosystem II assembly or activity but is needed for optimal photosystem II function.",
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T1 - Cloning of the psbK gene from synechocystis sp. PCC 6803 and characterization of photosystem II in mutants lacking PSII-K

AU - Ikeuchi, Masahiko

AU - Eggers, Beth

AU - Shen, Gaozhong

AU - Webber, Andrew

AU - Yu, Jiujiang

AU - Hirano, Atsushi

AU - Inoue, Yorinao

AU - Vermaas, Willem

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N2 - We cloned and sequenced the psbK gene, coding for a small photosystem II component (PSII-K), from the transformable cyanobacterium, Synechocystis sp. PCC 6803, and determined the N-terminal sequence of mature PSII-K. The psbK gene product is processed by cleaving off eight amino acid residues from the N terminus. A mutant lacking psbK was constructed; this mutant grew photoautotrophically, but its growth rate was reduced. The number of photosystem II reaction centers on a chlorophyll basis was decreased by less than a factor of 2 in the psbK-deletion mutant. In Synechocystis sp. PCC 6803, the psbK gene is transcribed as a single gene and is not part of an operon. Single-site mutations were introduced into psbK leading to early termination or deletion of the presequence. The phenotype of these mutants strongly resembles that of the psbK deletion mutant, indicating that indeed the change in phenotype in the deletion mutant is directly correlated with PSII-K. PSII-K is not essential for photosystem II assembly or activity but is needed for optimal photosystem II function.

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