Cloning and transfer of the Salmonella pathogenicity island 2 type III secretion system for studies of a range of gram-negative genera

James W. Wilson, Clint Coleman, Cheryl Nickerson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The engineering of bacterial strains with specific phenotypes frequently requires the use of blocks or "cassettes" of genes that act together to perform a desired function. The potential benefits of utilizing type III secretion systems in this regard are becoming increasingly realized since these systems can be used to direct interactions with host cells for beneficial purposes such as vaccine development, anticancer therapies, and targeted protein delivery. However, convenient methods to clone and transfer type III secretion systems for studies of a range of different types of bacteria are lacking. In addition to functional applications, such methods would also reveal important information about the evolution of a given type III secretion system, such as its ability to be expressed and functional outside of the strain of origin. We describe here the cloning of the Salmonella enterica serovar Typhimurium pathogenicity island 2 (SPI-2) type III secretion system onto a vector that can be easily transferred to a range of gram-negative bacterial genera. We found that expression of the cloned SPI-2 system in different Gammaproteobacteria and Alphaproteobacteria (as monitored by SseB protein levels) is dependent on the bacterial strain and growth medium. We also demonstrate that the cloned system is functional for secretion, can direct interactions with macrophages, and can be used as a novel tool to analyze the predicted interaction of SseB with host cells. This work provides a foundation for future applications where the cloned SPI-2 region (or other cloned type III systems) can provide a desired function to an engineered gram-negative strain.

Original languageEnglish (US)
Pages (from-to)5911-5918
Number of pages8
JournalApplied and Environmental Microbiology
Volume73
Issue number18
DOIs
StatePublished - Sep 2007

Fingerprint

pathogenicity islands
Genomic Islands
type III secretion system
pathogenicity
Salmonella
secretion
Organism Cloning
molecular cloning
serotypes
Alphaproteobacteria
Gammaproteobacteria
Salmonella enterica
alpha-Proteobacteria
gamma-Proteobacteria
vaccine development
application methods
Salmonella Typhimurium
protein
vaccine
engineering

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Cloning and transfer of the Salmonella pathogenicity island 2 type III secretion system for studies of a range of gram-negative genera. / Wilson, James W.; Coleman, Clint; Nickerson, Cheryl.

In: Applied and Environmental Microbiology, Vol. 73, No. 18, 09.2007, p. 5911-5918.

Research output: Contribution to journalArticle

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