TY - JOUR
T1 - Characterization and taxonomic implications of the rRNA genes of Mycobacterium leprae
AU - Sela, S.
AU - Clark-Curtiss, J. E.
AU - Bercovier, H.
PY - 1989
Y1 - 1989
N2 - The number of rRNA genes of Mycobacterium leprae was determined by restriction analysis of M. leprae total chromosomal DNA. A single set of rRNA genes was found. This set was subcloned from a cosmid library of M. leprae DNA into pUC13 and was characterized by restriction analysis and hybridization with Escherichia coli rRNA genes. The 16S, 23S, and 5S genes of M. leprae were clustered on a 5.3-kilobase DNA fragment. On one hand, restriction analysis of the set of rRNA genes showed the uniqueness of M. leprae among mycobacteria, but on the other hand, it suggested that M. leprae strains of several origins are very much alike. Quantitative hybridization studies between M. leprae rDNA and total DNA of various bacteria demonstrated a close relatedness between M. leprae and corynebacteria, nocardia, and mycobacteria, especially Mycobacterium tuberculosis.
AB - The number of rRNA genes of Mycobacterium leprae was determined by restriction analysis of M. leprae total chromosomal DNA. A single set of rRNA genes was found. This set was subcloned from a cosmid library of M. leprae DNA into pUC13 and was characterized by restriction analysis and hybridization with Escherichia coli rRNA genes. The 16S, 23S, and 5S genes of M. leprae were clustered on a 5.3-kilobase DNA fragment. On one hand, restriction analysis of the set of rRNA genes showed the uniqueness of M. leprae among mycobacteria, but on the other hand, it suggested that M. leprae strains of several origins are very much alike. Quantitative hybridization studies between M. leprae rDNA and total DNA of various bacteria demonstrated a close relatedness between M. leprae and corynebacteria, nocardia, and mycobacteria, especially Mycobacterium tuberculosis.
UR - http://www.scopus.com/inward/record.url?scp=0024538007&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024538007&partnerID=8YFLogxK
U2 - 10.1128/jb.171.1.70-73.1989
DO - 10.1128/jb.171.1.70-73.1989
M3 - Article
C2 - 2644213
AN - SCOPUS:0024538007
SN - 0021-9193
VL - 171
SP - 70
EP - 73
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 1
ER -