Abstract

This study reports the dechlorination characteristics of acclimated biomass, high-throughput sequencing of the 16Sribosomal RNA (rRNA) gene harbored by the microorganisms, and analysis of the relation between the dechlorination function and community structure. Anaerobic 2,4,6-trichlorophenol (TCP)-dechlorinating biomass was acclimated from active sludge, and it showed a high TCP-dechlorination rate. It dechlorinated TCP only at the ortho-position, with 4-chlorophenol as the terminal product. Illumina Hiseq sequencing analyses of a 16SrRNA gene amplicon indicated that the abundance of the phyla Bacteroidetes and Firmicutes doubled in the acclimation process. Compared with that in raw sludge, Betaproteobacteria disappeared from acclimated biomass, and Deltaproteobacteria was replaced by sulfate-reducing bacteria (SRB) including Desulfobulbus, Desulfovibrio, Desulfomicrobium, and Syntrophus. Syntrophomonas of the phylum Firmicutes predominated (abundance 6.7%), and it was responsible for the high efficacy of dechlorination. Dechlorinators included Dechloromonas, Clostridium, SRB and Geobacter. The emergence of SRB provided microbiological evidence of the sulfate-reducing ability of the dechlorinating biomass. Propionicimonas was discovered during this study, and it is known to produce vitamin B12, which is an essential factor for reductive dehalogenase enzymes. The core microbiome resulted in the high dechlorination rate.

Original languageEnglish (US)
Pages (from-to)306-313
Number of pages8
JournalJournal of Cleaner Production
Volume197
DOIs
StatePublished - Oct 1 2018

Fingerprint

Dechlorination
Sewage sludge
dechlorination
microbial community
Throughput
sulfate-reducing bacterium
Biomass
Bacteria
biomass
Genes
sludge
Clostridium
gene
Vitamins
chlorophenol
vitamin
acclimation
Microorganisms
community structure
Enzymes

Keywords

  • 2,4,6-Trichlorophenol
  • High-throughput sequencing
  • Microbial community
  • Reductive dechlorination
  • Sewage sludge

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Environmental Science(all)
  • Strategy and Management
  • Industrial and Manufacturing Engineering

Cite this

Characterization and high-throughput sequencing of a trichlorophenol-dechlorinating microbial community acclimated from sewage sludge. / Song, Jiaxiu; Chen, Lanjuan; Chen, Huidong; Sheng, Fanfan; Xing, Defeng; Li, Ling; Zhang, Yongming; Rittmann, Bruce.

In: Journal of Cleaner Production, Vol. 197, 01.10.2018, p. 306-313.

Research output: Contribution to journalArticle

Song, Jiaxiu ; Chen, Lanjuan ; Chen, Huidong ; Sheng, Fanfan ; Xing, Defeng ; Li, Ling ; Zhang, Yongming ; Rittmann, Bruce. / Characterization and high-throughput sequencing of a trichlorophenol-dechlorinating microbial community acclimated from sewage sludge. In: Journal of Cleaner Production. 2018 ; Vol. 197. pp. 306-313.
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AU - Sheng, Fanfan

AU - Xing, Defeng

AU - Li, Ling

AU - Zhang, Yongming

AU - Rittmann, Bruce

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AB - This study reports the dechlorination characteristics of acclimated biomass, high-throughput sequencing of the 16Sribosomal RNA (rRNA) gene harbored by the microorganisms, and analysis of the relation between the dechlorination function and community structure. Anaerobic 2,4,6-trichlorophenol (TCP)-dechlorinating biomass was acclimated from active sludge, and it showed a high TCP-dechlorination rate. It dechlorinated TCP only at the ortho-position, with 4-chlorophenol as the terminal product. Illumina Hiseq sequencing analyses of a 16SrRNA gene amplicon indicated that the abundance of the phyla Bacteroidetes and Firmicutes doubled in the acclimation process. Compared with that in raw sludge, Betaproteobacteria disappeared from acclimated biomass, and Deltaproteobacteria was replaced by sulfate-reducing bacteria (SRB) including Desulfobulbus, Desulfovibrio, Desulfomicrobium, and Syntrophus. Syntrophomonas of the phylum Firmicutes predominated (abundance 6.7%), and it was responsible for the high efficacy of dechlorination. Dechlorinators included Dechloromonas, Clostridium, SRB and Geobacter. The emergence of SRB provided microbiological evidence of the sulfate-reducing ability of the dechlorinating biomass. Propionicimonas was discovered during this study, and it is known to produce vitamin B12, which is an essential factor for reductive dehalogenase enzymes. The core microbiome resulted in the high dechlorination rate.

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