Abstract
This study reports the dechlorination characteristics of acclimated biomass, high-throughput sequencing of the 16Sribosomal RNA (rRNA) gene harbored by the microorganisms, and analysis of the relation between the dechlorination function and community structure. Anaerobic 2,4,6-trichlorophenol (TCP)-dechlorinating biomass was acclimated from active sludge, and it showed a high TCP-dechlorination rate. It dechlorinated TCP only at the ortho-position, with 4-chlorophenol as the terminal product. Illumina Hiseq sequencing analyses of a 16SrRNA gene amplicon indicated that the abundance of the phyla Bacteroidetes and Firmicutes doubled in the acclimation process. Compared with that in raw sludge, Betaproteobacteria disappeared from acclimated biomass, and Deltaproteobacteria was replaced by sulfate-reducing bacteria (SRB) including Desulfobulbus, Desulfovibrio, Desulfomicrobium, and Syntrophus. Syntrophomonas of the phylum Firmicutes predominated (abundance 6.7%), and it was responsible for the high efficacy of dechlorination. Dechlorinators included Dechloromonas, Clostridium, SRB and Geobacter. The emergence of SRB provided microbiological evidence of the sulfate-reducing ability of the dechlorinating biomass. Propionicimonas was discovered during this study, and it is known to produce vitamin B12, which is an essential factor for reductive dehalogenase enzymes. The core microbiome resulted in the high dechlorination rate.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 306-313 |
| Number of pages | 8 |
| Journal | Journal of Cleaner Production |
| Volume | 197 |
| DOIs | |
| State | Published - Oct 1 2018 |
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Keywords
- 2,4,6-Trichlorophenol
- High-throughput sequencing
- Microbial community
- Reductive dechlorination
- Sewage sludge
ASJC Scopus subject areas
- Renewable Energy, Sustainability and the Environment
- Environmental Science(all)
- Strategy and Management
- Industrial and Manufacturing Engineering
Cite this
Characterization and high-throughput sequencing of a trichlorophenol-dechlorinating microbial community acclimated from sewage sludge. / Song, Jiaxiu; Chen, Lanjuan; Chen, Huidong; Sheng, Fanfan; Xing, Defeng; Li, Ling; Zhang, Yongming; Rittmann, Bruce.
In: Journal of Cleaner Production, Vol. 197, 01.10.2018, p. 306-313.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization and high-throughput sequencing of a trichlorophenol-dechlorinating microbial community acclimated from sewage sludge
AU - Song, Jiaxiu
AU - Chen, Lanjuan
AU - Chen, Huidong
AU - Sheng, Fanfan
AU - Xing, Defeng
AU - Li, Ling
AU - Zhang, Yongming
AU - Rittmann, Bruce
PY - 2018/10/1
Y1 - 2018/10/1
N2 - This study reports the dechlorination characteristics of acclimated biomass, high-throughput sequencing of the 16Sribosomal RNA (rRNA) gene harbored by the microorganisms, and analysis of the relation between the dechlorination function and community structure. Anaerobic 2,4,6-trichlorophenol (TCP)-dechlorinating biomass was acclimated from active sludge, and it showed a high TCP-dechlorination rate. It dechlorinated TCP only at the ortho-position, with 4-chlorophenol as the terminal product. Illumina Hiseq sequencing analyses of a 16SrRNA gene amplicon indicated that the abundance of the phyla Bacteroidetes and Firmicutes doubled in the acclimation process. Compared with that in raw sludge, Betaproteobacteria disappeared from acclimated biomass, and Deltaproteobacteria was replaced by sulfate-reducing bacteria (SRB) including Desulfobulbus, Desulfovibrio, Desulfomicrobium, and Syntrophus. Syntrophomonas of the phylum Firmicutes predominated (abundance 6.7%), and it was responsible for the high efficacy of dechlorination. Dechlorinators included Dechloromonas, Clostridium, SRB and Geobacter. The emergence of SRB provided microbiological evidence of the sulfate-reducing ability of the dechlorinating biomass. Propionicimonas was discovered during this study, and it is known to produce vitamin B12, which is an essential factor for reductive dehalogenase enzymes. The core microbiome resulted in the high dechlorination rate.
AB - This study reports the dechlorination characteristics of acclimated biomass, high-throughput sequencing of the 16Sribosomal RNA (rRNA) gene harbored by the microorganisms, and analysis of the relation between the dechlorination function and community structure. Anaerobic 2,4,6-trichlorophenol (TCP)-dechlorinating biomass was acclimated from active sludge, and it showed a high TCP-dechlorination rate. It dechlorinated TCP only at the ortho-position, with 4-chlorophenol as the terminal product. Illumina Hiseq sequencing analyses of a 16SrRNA gene amplicon indicated that the abundance of the phyla Bacteroidetes and Firmicutes doubled in the acclimation process. Compared with that in raw sludge, Betaproteobacteria disappeared from acclimated biomass, and Deltaproteobacteria was replaced by sulfate-reducing bacteria (SRB) including Desulfobulbus, Desulfovibrio, Desulfomicrobium, and Syntrophus. Syntrophomonas of the phylum Firmicutes predominated (abundance 6.7%), and it was responsible for the high efficacy of dechlorination. Dechlorinators included Dechloromonas, Clostridium, SRB and Geobacter. The emergence of SRB provided microbiological evidence of the sulfate-reducing ability of the dechlorinating biomass. Propionicimonas was discovered during this study, and it is known to produce vitamin B12, which is an essential factor for reductive dehalogenase enzymes. The core microbiome resulted in the high dechlorination rate.
KW - 2,4,6-Trichlorophenol
KW - High-throughput sequencing
KW - Microbial community
KW - Reductive dechlorination
KW - Sewage sludge
UR - http://www.scopus.com/inward/record.url?scp=85049341432&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049341432&partnerID=8YFLogxK
U2 - 10.1016/j.jclepro.2018.06.061
DO - 10.1016/j.jclepro.2018.06.061
M3 - Article
VL - 197
SP - 306
EP - 313
JO - Journal of Cleaner Production
JF - Journal of Cleaner Production
SN - 0959-6526
ER -