CHAPTER 2: Imaging Protein Dynamics by XFELs

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The advances, opportunities and challenges during the first 7 years of structural biology using X-ray lasers are reviewed and their historical context provided. The main focus is on the achievements and prospects for imaging protein dynamics at near-atomic spatial resolution under native and controlled chemical conditions, and a summary of the many approaches to this aim is given. Radiation damage, comparisons of XFEL and synchrotron work, single particle diffraction, fast solution scattering, pump-probe studies on photosensitive proteins, mixing jets, caged molecules, pH jump and other reaction initiation methods, and the thermodynamics of molecular machines are all discussed, in addition to data analysis methods for all the instrumental modes. The ability of the XFEL to separate chemical reaction effects in dynamical imaging from radiation-induced effects (by minimizing these), while imaging at physiological temperatures and in the correct heat bath required for molecular machines is highlighted.

Original languageEnglish (US)
Title of host publicationThermoelectric Materials and Devices
PublisherRoyal Society of Chemistry
Pages47-69
Number of pages23
Volume2017-January
Edition18
DOIs
StatePublished - 2017

Publication series

NameRSC Energy and Environment Series
Number18
Volume2017-January
ISSN (Print)2044-0774
ISSN (Electronic)2044-0782

Fingerprint

Proteins
Imaging techniques
radiation damage
protein
chemical reaction
diffraction
X ray lasers
pump
spatial resolution
thermodynamics
laser
Radiation damage
scattering
probe
Synchrotrons
Chemical reactions
Diffraction
Pumps
Thermodynamics
Scattering

ASJC Scopus subject areas

  • Global and Planetary Change
  • Environmental Chemistry
  • Energy(all)

Cite this

Spence, J. (2017). CHAPTER 2: Imaging Protein Dynamics by XFELs. In Thermoelectric Materials and Devices (18 ed., Vol. 2017-January, pp. 47-69). (RSC Energy and Environment Series; Vol. 2017-January, No. 18). Royal Society of Chemistry. https://doi.org/10.1039/9781782624097-00045

CHAPTER 2 : Imaging Protein Dynamics by XFELs. / Spence, John.

Thermoelectric Materials and Devices. Vol. 2017-January 18. ed. Royal Society of Chemistry, 2017. p. 47-69 (RSC Energy and Environment Series; Vol. 2017-January, No. 18).

Research output: Chapter in Book/Report/Conference proceedingChapter

Spence, J 2017, CHAPTER 2: Imaging Protein Dynamics by XFELs. in Thermoelectric Materials and Devices. 18 edn, vol. 2017-January, RSC Energy and Environment Series, no. 18, vol. 2017-January, Royal Society of Chemistry, pp. 47-69. https://doi.org/10.1039/9781782624097-00045
Spence J. CHAPTER 2: Imaging Protein Dynamics by XFELs. In Thermoelectric Materials and Devices. 18 ed. Vol. 2017-January. Royal Society of Chemistry. 2017. p. 47-69. (RSC Energy and Environment Series; 18). https://doi.org/10.1039/9781782624097-00045
Spence, John. / CHAPTER 2 : Imaging Protein Dynamics by XFELs. Thermoelectric Materials and Devices. Vol. 2017-January 18. ed. Royal Society of Chemistry, 2017. pp. 47-69 (RSC Energy and Environment Series; 18).
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