Cas9 gRNA engineering for genome editing, activation and repression

Samira Kiani, Alejandro Chavez, Marcelle Tuttle, Richard N. Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian, Benjamin W. Pruitt, Jacob Beal, Suhani Vora, Joanna Buchthal, Emma J.K. Kowal, Mohammad R. Ebrahimkhani, James J. Collins, Ron Weiss, George Church

Research output: Contribution to journalReview articlepeer-review

204 Scopus citations


We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.

Original languageEnglish (US)
Pages (from-to)1051-1054
Number of pages4
JournalNature Methods
Issue number11
StatePublished - Nov 1 2015

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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