Cas9 gRNA engineering for genome editing, activation and repression

Samira Kiani, Alejandro Chavez, Marcelle Tuttle, Richard N. Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian, Benjamin W. Pruitt, Jacob Beal, Suhani Vora, Joanna Buchthal, Emma J K Kowal, Mohammad Ebrahimkhani, James J. Collins, Ron Weiss, George Church

Research output: Contribution to journalReview article

135 Scopus citations


We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.

Original languageEnglish (US)
Pages (from-to)1051-1054
Number of pages4
JournalNature Methods
Issue number11
StatePublished - Nov 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

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  • Cite this

    Kiani, S., Chavez, A., Tuttle, M., Hall, R. N., Chari, R., Ter-Ovanesyan, D., Qian, J., Pruitt, B. W., Beal, J., Vora, S., Buchthal, J., Kowal, E. J. K., Ebrahimkhani, M., Collins, J. J., Weiss, R., & Church, G. (2015). Cas9 gRNA engineering for genome editing, activation and repression. Nature Methods, 12(11), 1051-1054.