Cas9 gRNA engineering for genome editing, activation and repression

Samira Kiani, Alejandro Chavez, Marcelle Tuttle, Richard N. Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian, Benjamin W. Pruitt, Jacob Beal, Suhani Vora, Joanna Buchthal, Emma J K Kowal, Mohammad Ebrahimkhani, James J. Collins, Ron Weiss, George Church

Research output: Contribution to journalReview article

124 Citations (Scopus)

Abstract

We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.

Original languageEnglish (US)
Pages (from-to)1051-1054
Number of pages4
JournalNature Methods
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2015
Externally publishedYes

Fingerprint

Guide RNA
Genes
Chemical activation
Proteins
Engineers
Networks (circuits)
Gene Editing

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Kiani, S., Chavez, A., Tuttle, M., Hall, R. N., Chari, R., Ter-Ovanesyan, D., ... Church, G. (2015). Cas9 gRNA engineering for genome editing, activation and repression. Nature Methods, 12(11), 1051-1054. https://doi.org/10.1038/nmeth.3580

Cas9 gRNA engineering for genome editing, activation and repression. / Kiani, Samira; Chavez, Alejandro; Tuttle, Marcelle; Hall, Richard N.; Chari, Raj; Ter-Ovanesyan, Dmitry; Qian, Jason; Pruitt, Benjamin W.; Beal, Jacob; Vora, Suhani; Buchthal, Joanna; Kowal, Emma J K; Ebrahimkhani, Mohammad; Collins, James J.; Weiss, Ron; Church, George.

In: Nature Methods, Vol. 12, No. 11, 01.11.2015, p. 1051-1054.

Research output: Contribution to journalReview article

Kiani, S, Chavez, A, Tuttle, M, Hall, RN, Chari, R, Ter-Ovanesyan, D, Qian, J, Pruitt, BW, Beal, J, Vora, S, Buchthal, J, Kowal, EJK, Ebrahimkhani, M, Collins, JJ, Weiss, R & Church, G 2015, 'Cas9 gRNA engineering for genome editing, activation and repression', Nature Methods, vol. 12, no. 11, pp. 1051-1054. https://doi.org/10.1038/nmeth.3580
Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D et al. Cas9 gRNA engineering for genome editing, activation and repression. Nature Methods. 2015 Nov 1;12(11):1051-1054. https://doi.org/10.1038/nmeth.3580
Kiani, Samira ; Chavez, Alejandro ; Tuttle, Marcelle ; Hall, Richard N. ; Chari, Raj ; Ter-Ovanesyan, Dmitry ; Qian, Jason ; Pruitt, Benjamin W. ; Beal, Jacob ; Vora, Suhani ; Buchthal, Joanna ; Kowal, Emma J K ; Ebrahimkhani, Mohammad ; Collins, James J. ; Weiss, Ron ; Church, George. / Cas9 gRNA engineering for genome editing, activation and repression. In: Nature Methods. 2015 ; Vol. 12, No. 11. pp. 1051-1054.
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