Cas9 gRNA engineering for genome editing, activation and repression

Samira Kiani; Alejandro Chavez; Marcelle Tuttle; Richard N. Hall; Raj Chari; Dmitry Ter-Ovanesyan; Jason Qian; Benjamin W. Pruitt; Jacob Beal; Suhani Vora; Joanna Buchthal; Emma J.K. Kowal; Mohammad R. Ebrahimkhani; James J. Collins; Ron Weiss; George Church

doi:10.1038/nmeth.3580

Cas9 gRNA engineering for genome editing, activation and repression

Samira Kiani, Alejandro Chavez, Marcelle Tuttle, Richard N. Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian, Benjamin W. Pruitt, Jacob Beal, Suhani Vora, Joanna Buchthal, Emma J.K. Kowal, Mohammad R. Ebrahimkhani, James J. Collins, Ron Weiss, George Church

Research output: Contribution to journal › Review article › peer-review

240 Scopus citations

Abstract

We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.

Original language	English (US)
Pages (from-to)	1051-1054
Number of pages	4
Journal	Nature Methods
Volume	12
Issue number	11
DOIs	https://doi.org/10.1038/nmeth.3580
State	Published - Nov 1 2015

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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title = "Cas9 gRNA engineering for genome editing, activation and repression",

abstract = "We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.",

author = "Samira Kiani and Alejandro Chavez and Marcelle Tuttle and Hall, {Richard N.} and Raj Chari and Dmitry Ter-Ovanesyan and Jason Qian and Pruitt, {Benjamin W.} and Jacob Beal and Suhani Vora and Joanna Buchthal and Kowal, {Emma J.K.} and Ebrahimkhani, {Mohammad R.} and Collins, {James J.} and Ron Weiss and George Church",

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AU - Kiani, Samira

AU - Chavez, Alejandro

AU - Tuttle, Marcelle

AU - Hall, Richard N.

AU - Chari, Raj

AU - Ter-Ovanesyan, Dmitry

AU - Qian, Jason

AU - Pruitt, Benjamin W.

AU - Beal, Jacob

AU - Vora, Suhani

AU - Buchthal, Joanna

AU - Kowal, Emma J.K.

AU - Ebrahimkhani, Mohammad R.

AU - Collins, James J.

AU - Weiss, Ron

AU - Church, George

PY - 2015/11/1

Y1 - 2015/11/1

N2 - We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.

AB - We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.

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DO - 10.1038/nmeth.3580

M3 - Review article

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VL - 12

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JO - Nature Methods

JF - Nature Methods

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ER -

Cas9 gRNA engineering for genome editing, activation and repression

Abstract

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