Bryostatins: Potent, new mitogens that mimic phorbol ester tumor promoters

Jeffrey B. Smith, Lucinda Smith, George Pettit

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Bryostatins (2 ng/ml), when combined with insulin in serum-free culture medium, are strongly mitogenic for Swiss 3T3 cells that have been arrested in the G1 G0 phase of the cell cycle. The mitogenic effect of the bryostatins is similar to that of 12-0-decanoylphorbol-13-acetate (TPA). A prior treatment of the cultures with TPA eliminated the mitogenic response to bryostatin and to a second addition of TPA. Conversely, a prior treatment of the cultures with bryostatin eliminated the mitogenic response to TPA. Bryostatin potently inhibited the binding of [3H]phorbol dibutyrate to a high affinity receptor in the cells. The findings suggest that the bryostatins and TPA act via the same receptor, possibly protein kinase C.

Original languageEnglish (US)
Pages (from-to)939-945
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume132
Issue number3
DOIs
StatePublished - Nov 15 1985

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Bryostatins
Phorbol Esters
Mitogens
Carcinogens
Swiss 3T3 Cells
Cell Cycle Resting Phase
Serum-Free Culture Media
G1 Phase
Cell Cycle
Acetates
Cells
Insulin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Bryostatins : Potent, new mitogens that mimic phorbol ester tumor promoters. / Smith, Jeffrey B.; Smith, Lucinda; Pettit, George.

In: Biochemical and Biophysical Research Communications, Vol. 132, No. 3, 15.11.1985, p. 939-945.

Research output: Contribution to journalArticle

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