Bryostatins: Potent, new mitogens that mimic phorbol ester tumor promoters

Jeffrey B. Smith, Lucinda Smith, George Pettit

Research output: Contribution to journalArticlepeer-review

130 Scopus citations

Abstract

Bryostatins (2 ng/ml), when combined with insulin in serum-free culture medium, are strongly mitogenic for Swiss 3T3 cells that have been arrested in the G1 G0 phase of the cell cycle. The mitogenic effect of the bryostatins is similar to that of 12-0-decanoylphorbol-13-acetate (TPA). A prior treatment of the cultures with TPA eliminated the mitogenic response to bryostatin and to a second addition of TPA. Conversely, a prior treatment of the cultures with bryostatin eliminated the mitogenic response to TPA. Bryostatin potently inhibited the binding of [3H]phorbol dibutyrate to a high affinity receptor in the cells. The findings suggest that the bryostatins and TPA act via the same receptor, possibly protein kinase C.

Original languageEnglish (US)
Pages (from-to)939-945
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume132
Issue number3
DOIs
StatePublished - Nov 15 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Bryostatins: Potent, new mitogens that mimic phorbol ester tumor promoters'. Together they form a unique fingerprint.

Cite this