Abstract
Serial femtosecond crystallography (SFX) laser pulses facilitate the structure determination of smaller crystals than can generally be studied by traditional synchrotron crystallographic methods. One limiting factor of SFX is the large volume of crystal suspension needed to obtain a full data set allowing structure determination. The current method of continuous sample stream injection into an X-ray free electron laser (XFEL) requires milliliters of sample and wastes the majority of the protein crystals grown under time consuming conditions. We propose the utilization of aqueous droplets as an efficient method for protein crystal delivery into an XFEL for structure determination by SFX.
| Original language | English (US) |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1374-1376 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| State | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: Oct 25 2015 → Oct 29 2015 |
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country | Korea, Republic of |
| City | Gyeongju |
| Period | 10/25/15 → 10/29/15 |
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Keywords
- Droplets
- Microfluidics
- X-ray Crystallography
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser. / Echelmeier, A.; Nelson, G.; Abdallah, B. G.; James, D.; Roy-Chowdhury, S.; Tolstikova, A.; Mariani, V.; Kirian, Richard; Oberthüer, D.; Dörner, K.; Fromme, Petra; Chapman, H. N.; Weierstall, Uwe; Spence, John; Ros, Alexandra.
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 1374-1376.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser
AU - Echelmeier, A.
AU - Nelson, G.
AU - Abdallah, B. G.
AU - James, D.
AU - Roy-Chowdhury, S.
AU - Tolstikova, A.
AU - Mariani, V.
AU - Kirian, Richard
AU - Oberthüer, D.
AU - Dörner, K.
AU - Fromme, Petra
AU - Chapman, H. N.
AU - Weierstall, Uwe
AU - Spence, John
AU - Ros, Alexandra
PY - 2015
Y1 - 2015
N2 - Serial femtosecond crystallography (SFX) laser pulses facilitate the structure determination of smaller crystals than can generally be studied by traditional synchrotron crystallographic methods. One limiting factor of SFX is the large volume of crystal suspension needed to obtain a full data set allowing structure determination. The current method of continuous sample stream injection into an X-ray free electron laser (XFEL) requires milliliters of sample and wastes the majority of the protein crystals grown under time consuming conditions. We propose the utilization of aqueous droplets as an efficient method for protein crystal delivery into an XFEL for structure determination by SFX.
AB - Serial femtosecond crystallography (SFX) laser pulses facilitate the structure determination of smaller crystals than can generally be studied by traditional synchrotron crystallographic methods. One limiting factor of SFX is the large volume of crystal suspension needed to obtain a full data set allowing structure determination. The current method of continuous sample stream injection into an X-ray free electron laser (XFEL) requires milliliters of sample and wastes the majority of the protein crystals grown under time consuming conditions. We propose the utilization of aqueous droplets as an efficient method for protein crystal delivery into an XFEL for structure determination by SFX.
KW - Droplets
KW - Microfluidics
KW - X-ray Crystallography
UR - http://www.scopus.com/inward/record.url?scp=84973622825&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973622825&partnerID=8YFLogxK
M3 - Conference contribution
SP - 1374
EP - 1376
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
ER -