@inproceedings{c2beaf57b15541698c770fe12f59a423,
title = "Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser",
abstract = "Serial femtosecond crystallography (SFX) laser pulses facilitate the structure determination of smaller crystals than can generally be studied by traditional synchrotron crystallographic methods. One limiting factor of SFX is the large volume of crystal suspension needed to obtain a full data set allowing structure determination. The current method of continuous sample stream injection into an X-ray free electron laser (XFEL) requires milliliters of sample and wastes the majority of the protein crystals grown under time consuming conditions. We propose the utilization of aqueous droplets as an efficient method for protein crystal delivery into an XFEL for structure determination by SFX.",
keywords = "Droplets, Microfluidics, X-ray Crystallography",
author = "A. Echelmeier and G. Nelson and Abdallah, {B. G.} and D. James and S. Roy-Chowdhury and A. Tolstikova and V. Mariani and Richard Kirian and D. Oberth{\"u}er and K. D{\"o}rner and Petra Fromme and Chapman, {H. N.} and Uwe Weierstall and John Spence and Alexandra Ros",
note = "Funding Information: This work was supported by the STC Program of the National Science Foundation through BioXFEL under Agreement No. 1231306 and the National Institutes of Health Award No. R01GM095583. Portions of this research were carried out at the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory. LCLS is an Office of Science User Facility operated for the U.S. Department of Energy Office of Science by Stanford University. We thank Andrew Aquila, Mark Hunter, and S{\'e}bastien Boutet for support during data collection at the Coherent X-ray Imaging (CXI) instrument at LCLS. Publisher Copyright: {\textcopyright} 15CBMS-0001.; 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 ; Conference date: 25-10-2015 Through 29-10-2015",
year = "2015",
language = "English (US)",
series = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "1374--1376",
booktitle = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}