Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser

A. Echelmeier; G. Nelson; B. G. Abdallah; D. James; S. Roy-Chowdhury; A. Tolstikova; V. Mariani; Richard Kirian; D. Oberthüer; K. Dörner; Petra Fromme; H. N. Chapman; Uwe Weierstall; John Spence; Alexandra Ros

Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser

A. Echelmeier, G. Nelson, B. G. Abdallah, D. James, S. Roy-Chowdhury, A. Tolstikova, V. Mariani, Richard Kirian, D. Oberthüer, K. Dörner, Petra Fromme, H. N. Chapman, Uwe Weierstall, John Spence, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Scopus citations

Abstract

Serial femtosecond crystallography (SFX) laser pulses facilitate the structure determination of smaller crystals than can generally be studied by traditional synchrotron crystallographic methods. One limiting factor of SFX is the large volume of crystal suspension needed to obtain a full data set allowing structure determination. The current method of continuous sample stream injection into an X-ray free electron laser (XFEL) requires milliliters of sample and wastes the majority of the protein crystals grown under time consuming conditions. We propose the utilization of aqueous droplets as an efficient method for protein crystal delivery into an XFEL for structure determination by SFX.

Original language	English (US)
Title of host publication	MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1374-1376
Number of pages	3
ISBN (Electronic)	9780979806483
State	Published - 2015
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: Oct 25 2015 → Oct 29 2015

Other

Other	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country	Korea, Republic of
City	Gyeongju
Period	10/25/15 → 10/29/15

Keywords

Droplets
Microfluidics
X-ray Crystallography

ASJC Scopus subject areas

Control and Systems Engineering

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Cite this

Echelmeier, A., Nelson, G., Abdallah, B. G., James, D., Roy-Chowdhury, S., Tolstikova, A., Mariani, V., Kirian, R., Oberthüer, D., Dörner, K., Fromme, P., Chapman, H. N., Weierstall, U., Spence, J., & Ros, A. (2015). Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1374-1376). Chemical and Biological Microsystems Society.

Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser. / Echelmeier, A.; Nelson, G.; Abdallah, B. G.; James, D.; Roy-Chowdhury, S.; Tolstikova, A.; Mariani, V.; Kirian, Richard; Oberthüer, D.; Dörner, K.; Fromme, Petra; Chapman, H. N.; Weierstall, Uwe ; Spence, John ; Ros, Alexandra.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 1374-1376.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Echelmeier, A, Nelson, G, Abdallah, BG, James, D, Roy-Chowdhury, S, Tolstikova, A, Mariani, V, Kirian, R, Oberthüer, D, Dörner, K, Fromme, P, Chapman, HN, Weierstall, U , Spence, J & Ros, A 2015, Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1374-1376, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 10/25/15.

Echelmeier A, Nelson G, Abdallah BG, James D, Roy-Chowdhury S, Tolstikova A et al. Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2015. p. 1374-1376

Echelmeier, A. ; Nelson, G. ; Abdallah, B. G. ; James, D. ; Roy-Chowdhury, S. ; Tolstikova, A. ; Mariani, V. ; Kirian, Richard ; Oberthüer, D. ; Dörner, K. ; Fromme, Petra ; Chapman, H. N. ; Weierstall, Uwe ; Spence, John ; Ros, Alexandra. / Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. pp. 1374-1376

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abstract = "Serial femtosecond crystallography (SFX) laser pulses facilitate the structure determination of smaller crystals than can generally be studied by traditional synchrotron crystallographic methods. One limiting factor of SFX is the large volume of crystal suspension needed to obtain a full data set allowing structure determination. The current method of continuous sample stream injection into an X-ray free electron laser (XFEL) requires milliliters of sample and wastes the majority of the protein crystals grown under time consuming conditions. We propose the utilization of aqueous droplets as an efficient method for protein crystal delivery into an XFEL for structure determination by SFX.",

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AU - Echelmeier, A.

AU - Nelson, G.

AU - Abdallah, B. G.

AU - James, D.

AU - Roy-Chowdhury, S.

AU - Tolstikova, A.

AU - Mariani, V.

AU - Kirian, Richard

AU - Oberthüer, D.

AU - Dörner, K.

AU - Fromme, Petra

AU - Chapman, H. N.

AU - Weierstall, Uwe

AU - Spence, John

AU - Ros, Alexandra

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AB - Serial femtosecond crystallography (SFX) laser pulses facilitate the structure determination of smaller crystals than can generally be studied by traditional synchrotron crystallographic methods. One limiting factor of SFX is the large volume of crystal suspension needed to obtain a full data set allowing structure determination. The current method of continuous sample stream injection into an X-ray free electron laser (XFEL) requires milliliters of sample and wastes the majority of the protein crystals grown under time consuming conditions. We propose the utilization of aqueous droplets as an efficient method for protein crystal delivery into an XFEL for structure determination by SFX.

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KW - X-ray Crystallography

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