Biopsy preparation for flow analysis using microfabricated disaggregation blades

Andrew K. Miller, Matthew L. Stanton, Carissa A. Sanchez, Xiaohong Li, Sarah C. McQuaide, Brian J. Reid, Deirdre R. Meldrum, Thomas G. Paulson, Mark R. Holl

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An epithelial tissue biopsy processor that produces stained cell nuclei as its output using microfabricated disaggregation structures is presented. Stained cell nuclei produced by the processor can be used for a variety of diagnostic tests to assess the presence of cancer or the risk of developing cancer in the future. To better understand the biopsy disaggregation process, biopsies prepared by a highly skilled technician were examined using fluorescence microscopy at various stages of the disaggregation process. Quantitative analysis of nuclei preparations were performed using flow cytometry of the end product. General device architecture, microfabricated glass blade tissue mincing structures, manual disaggregation microscopy evaluation results, DNA content flow cytometry results, and plans for further device development are presented.

Original languageEnglish (US)
Title of host publicationProceedings of the 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005
Pages1-6
Number of pages6
DOIs
StatePublished - Dec 1 2005
Externally publishedYes
Event2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005 - Edmonton, Canada
Duration: Aug 1 2005Aug 2 2005

Publication series

NameProceedings of the 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005
Volume2005

Other

Other2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005
CountryCanada
CityEdmonton
Period8/1/058/2/05

Keywords

  • Flow cytometry
  • Laboratory automation
  • Microfluidics
  • Sample preparation
  • Tissue disaggregation

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Miller, A. K., Stanton, M. L., Sanchez, C. A., Li, X., McQuaide, S. C., Reid, B. J., Meldrum, D. R., Paulson, T. G., & Holl, M. R. (2005). Biopsy preparation for flow analysis using microfabricated disaggregation blades. In Proceedings of the 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005 (pp. 1-6). [1506736] (Proceedings of the 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005; Vol. 2005). https://doi.org/10.1109/COASE.2005.1506736