Bioanalysis in structured microfluidic systems

Alexandra Ros, Wibke Hellmich, Jan Regtmeier, Thanh Tu Duong, Dario Anselmetti

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Microfluidic and lab-on-a-chip devices have attracted widespread interest in separation sciences and bioanalysis. Recent designs in microfluidic devices extend common separation concepts by exploiting new phenomena for molecular dynamics on a length scale of 10 μm and below, giving rise to novel manipulation tools and nonintuitive phenomena for microseparations. Here, we focus on three very recent developments for bioseparations based on tailored microfluidic systems: Single cell navigation, trapping and steering with subsequent on-chip lysis, protein separation and LIF detection (Section 3.1), then we report dielectrophoretic trapping and separation of large DNA fragments in structured microfluidic devices (Section 3.2). Finally, a paradoxial migration phenomenon based on thermal fluctuations, periodically arranged microchannels and a biased alternating current electric field is presented in Section 3.3.

Original languageEnglish (US)
Pages (from-to)2651-2658
Number of pages8
JournalElectrophoresis
Volume27
Issue number13
DOIs
StatePublished - Jul 1 2006
Externally publishedYes

Keywords

  • DNA
  • Microfluidic device
  • Migration phenomena
  • Protein
  • Single cell analytics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Bioanalysis in structured microfluidic systems'. Together they form a unique fingerprint.

  • Cite this

    Ros, A., Hellmich, W., Regtmeier, J., Duong, T. T., & Anselmetti, D. (2006). Bioanalysis in structured microfluidic systems. Electrophoresis, 27(13), 2651-2658. https://doi.org/10.1002/elps.200500923