TY - JOUR
T1 - Binding of metal ions to melanin and their effects on the aerobic reactivity
AU - Hong, Lian
AU - Liu, Yan
AU - Simon, John D.
PY - 2004/11/1
Y1 - 2004/11/1
N2 - The binding of Mg(II), Ca(II), Zn(II), Cu(II) and Fe(III) to ETDA-washed Sepia melanin is quantified by inductively coupled plasma mass spectrometry. By monitoring the solution pH change associated with metal uptake, it is concluded that Mg(II), Ca(II) and Zn(II) bind to carboxylic acid groups in melanin, Cu(II) binds to hydroxyl (OH) groups and Fe(III) binds to OH or amine groups. The aerobic reactivity of melanins with different metal contents is analyzed by examining their ability to cause strand breaks in supercoiled pUC18 DNA. Cu(II)- and Fe(III)-enriched melanins induce the most damage. Hydroxyl radical, •OH, is proposed to be one of the reactive oxygen species responsible.
AB - The binding of Mg(II), Ca(II), Zn(II), Cu(II) and Fe(III) to ETDA-washed Sepia melanin is quantified by inductively coupled plasma mass spectrometry. By monitoring the solution pH change associated with metal uptake, it is concluded that Mg(II), Ca(II) and Zn(II) bind to carboxylic acid groups in melanin, Cu(II) binds to hydroxyl (OH) groups and Fe(III) binds to OH or amine groups. The aerobic reactivity of melanins with different metal contents is analyzed by examining their ability to cause strand breaks in supercoiled pUC18 DNA. Cu(II)- and Fe(III)-enriched melanins induce the most damage. Hydroxyl radical, •OH, is proposed to be one of the reactive oxygen species responsible.
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U2 - 10.1562/2004-05-17-RA-172.1
DO - 10.1562/2004-05-17-RA-172.1
M3 - Article
C2 - 15623333
AN - SCOPUS:12944303659
SN - 0031-8655
VL - 80
SP - 477
EP - 481
JO - Photochemistry and photobiology
JF - Photochemistry and photobiology
IS - 3
ER -