Benzoic and salicylic acid are the signaling molecules of Chlorella cells for improving cell growth

Liang Fu, Qingcheng Li, Congli Chen, Yueju Zhang, Yinglu Liu, Liang Xu, Yihan Zhou, Chengbin Li, Dandan Zhou, Bruce E. Rittmann

Research output: Contribution to journalArticlepeer-review

Abstract

Cell-to-cell communication regulates microalgae production via signaling molecules (SMs), but few microalgal SM species are known. Here, we document two new microalgae SMs, benzoic acid (BA) and salicylic acid (SA). Initially, crude SMs were extracted from a microalgae culture in which microalgae grew on heterotrophic-enriched phosphorus nutrition. The extracted SMs enhanced Chlorella growth by ∼72%, promoted nutrient uptake, and up-regulated the mitogen-activated protein-kinase signaling cascade. Fourier transform infrared and nuclear magnetic resonance analyses identified the putative SMs was aromatic carboxylic acids. BA and SA were identified using high-resolution mass spectrometry. BA and SA addition increased cell growth by ∼75% and ∼25%; and improved ATP production by ∼35% and ∼20%. Transcriptomic analysis showed that BA and SA were biosynthesized via CoA-dependent, non-oxidative pathway. The SMs upregulated TCA-cycle enzymes, which promoted carbon assimilation and activated DNA-replicating enzyme, so that accelerated cell division. This study identified two new SMs for microalgae cell communication and provides means to identify other SMs.

Original languageEnglish (US)
Article number129084
JournalChemosphere
Volume265
DOIs
StateAccepted/In press - 2020

Keywords

  • Benzoic acid
  • Cell-to-cell communication
  • Microalgae
  • Salicylic acid
  • Signaling molecules

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

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