Benthic bacterial diversity from freshwater tufas of the Iberian Range (Spain)

Hugo Beraldi-Campesi, Concepción Arenas-Abad, Ferran Garcia-Pichel, Omar Arellano-Aguilar, Luis Auqué, Marta Vázquez-Urbez, Carlos Sancho, Cinta Osácar, Silvia Ruiz-Velasco

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aiming to characterize the bacterial diversity of modern tufa systems of the Iberian Range (Spain), we surveyed the 16S rRNA gene sequence diversity from 24 sites within three rivers (Añamaza, Mesa and Piedra). These tufas record substantial calcareous growth under different physicochemical conditions and are part of an important, regional landscape-building system. The bacterial community structure and composition, richness and diversity were quantified from denaturing gradient gel electrophoresis fingerprints. Retrieved DNA sequences could be assigned to 10 bacterial phyla and included a variety of phototrophic and heterotrophic groups. Cyanobacteria, mainly filamentous taxa, constituted 43% of all the retrieved sequences, followed by Firmicutes (11%), Gammaproteobacteria (10%), Alphaproteobacteria (7%), Acidobacteria (6%), Bacteroidetes (5%), Betaproteobacteria (4%), Planctomycetes (4%), Actinobacteria (3%) and Deltaproteobacteria (2%). Diatom and Xanthophyceae chloroplast sequences were also detected. Physicochemical variables measured at each site were modelled with multivariate statistics. Principal component analyses yielded the highest variance for salinity-related variables (conductivity; Na +, Cl - and SO4 2- concentrations), which correlated negatively and significantly with diversity indices. However, the highest variance explained by individual principal components was relatively low (< 34%). Overall, we show that these young fluvial tufas are inhabited by a large variety of bacteria in diverse and widespread communities.

Original languageEnglish (US)
Pages (from-to)363-379
Number of pages17
JournalFEMS Microbiology Ecology
Volume80
Issue number2
DOIs
StatePublished - May 2012

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Piedra
Acidobacteria
Deltaproteobacteria
Betaproteobacteria
Bacteroidetes
Alphaproteobacteria
Gammaproteobacteria
Bacterial Structures
Diatoms
tufa
Denaturing Gradient Gel Electrophoresis
Actinobacteria
Salinity
Dermatoglyphics
Cyanobacteria
Chloroplasts
Principal Component Analysis
Fresh Water
diversity index
rRNA Genes

Keywords

  • Bacterial diversity
  • Freshwater
  • Tufa

ASJC Scopus subject areas

  • Ecology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Beraldi-Campesi, H., Arenas-Abad, C., Garcia-Pichel, F., Arellano-Aguilar, O., Auqué, L., Vázquez-Urbez, M., ... Ruiz-Velasco, S. (2012). Benthic bacterial diversity from freshwater tufas of the Iberian Range (Spain). FEMS Microbiology Ecology, 80(2), 363-379. https://doi.org/10.1111/j.1574-6941.2012.01303.x

Benthic bacterial diversity from freshwater tufas of the Iberian Range (Spain). / Beraldi-Campesi, Hugo; Arenas-Abad, Concepción; Garcia-Pichel, Ferran; Arellano-Aguilar, Omar; Auqué, Luis; Vázquez-Urbez, Marta; Sancho, Carlos; Osácar, Cinta; Ruiz-Velasco, Silvia.

In: FEMS Microbiology Ecology, Vol. 80, No. 2, 05.2012, p. 363-379.

Research output: Contribution to journalArticle

Beraldi-Campesi, H, Arenas-Abad, C, Garcia-Pichel, F, Arellano-Aguilar, O, Auqué, L, Vázquez-Urbez, M, Sancho, C, Osácar, C & Ruiz-Velasco, S 2012, 'Benthic bacterial diversity from freshwater tufas of the Iberian Range (Spain)', FEMS Microbiology Ecology, vol. 80, no. 2, pp. 363-379. https://doi.org/10.1111/j.1574-6941.2012.01303.x
Beraldi-Campesi H, Arenas-Abad C, Garcia-Pichel F, Arellano-Aguilar O, Auqué L, Vázquez-Urbez M et al. Benthic bacterial diversity from freshwater tufas of the Iberian Range (Spain). FEMS Microbiology Ecology. 2012 May;80(2):363-379. https://doi.org/10.1111/j.1574-6941.2012.01303.x
Beraldi-Campesi, Hugo ; Arenas-Abad, Concepción ; Garcia-Pichel, Ferran ; Arellano-Aguilar, Omar ; Auqué, Luis ; Vázquez-Urbez, Marta ; Sancho, Carlos ; Osácar, Cinta ; Ruiz-Velasco, Silvia. / Benthic bacterial diversity from freshwater tufas of the Iberian Range (Spain). In: FEMS Microbiology Ecology. 2012 ; Vol. 80, No. 2. pp. 363-379.
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