Benthic bacterial diversity from freshwater tufas of the Iberian Range (Spain)

Hugo Beraldi-Campesi, Concepción Arenas-Abad, Ferran Garcia-Pichel, Omar Arellano-Aguilar, Luis Auqué, Marta Vázquez-Urbez, Carlos Sancho, Cinta Osácar, Silvia Ruiz-Velasco

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Aiming to characterize the bacterial diversity of modern tufa systems of the Iberian Range (Spain), we surveyed the 16S rRNA gene sequence diversity from 24 sites within three rivers (Añamaza, Mesa and Piedra). These tufas record substantial calcareous growth under different physicochemical conditions and are part of an important, regional landscape-building system. The bacterial community structure and composition, richness and diversity were quantified from denaturing gradient gel electrophoresis fingerprints. Retrieved DNA sequences could be assigned to 10 bacterial phyla and included a variety of phototrophic and heterotrophic groups. Cyanobacteria, mainly filamentous taxa, constituted 43% of all the retrieved sequences, followed by Firmicutes (11%), Gammaproteobacteria (10%), Alphaproteobacteria (7%), Acidobacteria (6%), Bacteroidetes (5%), Betaproteobacteria (4%), Planctomycetes (4%), Actinobacteria (3%) and Deltaproteobacteria (2%). Diatom and Xanthophyceae chloroplast sequences were also detected. Physicochemical variables measured at each site were modelled with multivariate statistics. Principal component analyses yielded the highest variance for salinity-related variables (conductivity; Na +, Cl - and SO4 2- concentrations), which correlated negatively and significantly with diversity indices. However, the highest variance explained by individual principal components was relatively low (< 34%). Overall, we show that these young fluvial tufas are inhabited by a large variety of bacteria in diverse and widespread communities.

Original languageEnglish (US)
Pages (from-to)363-379
Number of pages17
JournalFEMS microbiology ecology
Volume80
Issue number2
DOIs
StatePublished - May 1 2012

Keywords

  • Bacterial diversity
  • Freshwater
  • Tufa

ASJC Scopus subject areas

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

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    Beraldi-Campesi, H., Arenas-Abad, C., Garcia-Pichel, F., Arellano-Aguilar, O., Auqué, L., Vázquez-Urbez, M., Sancho, C., Osácar, C., & Ruiz-Velasco, S. (2012). Benthic bacterial diversity from freshwater tufas of the Iberian Range (Spain). FEMS microbiology ecology, 80(2), 363-379. https://doi.org/10.1111/j.1574-6941.2012.01303.x