Autonomous high throughput real time PCR platform for large scale environmental monitoring

Tathagata Ray, Andrew Hatch, Kelly Lintecum, Shih-Hui Chao, Cody Youngbull

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present the design and development of a continuous flow, semi-autonomous, quantitative Polymerase Chain Reaction (qPCR) system. This battery-powered system operates under programmable control and is able to detect many different primer probe combinations as is required for environmental analyses. Our design focuses on a system with re-usable components for low levels of consumables, automated fluid handling and low power consumption. It also maintains user control over essential parameters including sample volume, temperature, and cycle time. We use a two-phase emulsion system connected to a continuous flow, two-temperature zone cylindrical thermocycler. The fluorescence from each channel is excited using an LED coupled through a fiber optic bundle and emission detected using optical fiber coupling to a 64 channel PMT. Data collection and quantitative PCR analysis is performed using a laptop computer. This platform can be transported for in-situ field applications.

Original languageEnglish (US)
Title of host publicationTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Pages85-88
Number of pages4
StatePublished - Aug 9 2013
EventNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 - Washington, DC, United States
Duration: May 12 2013May 16 2013

Publication series

NameTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Volume3

Other

OtherNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
CountryUnited States
CityWashington, DC
Period5/12/135/16/13

Keywords

  • Continuous flow
  • Emulsion
  • High throughput
  • PMT
  • Real-time PCR

ASJC Scopus subject areas

  • Biotechnology

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  • Cite this

    Ray, T., Hatch, A., Lintecum, K., Chao, S-H., & Youngbull, C. (2013). Autonomous high throughput real time PCR platform for large scale environmental monitoring. In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 (pp. 85-88). (Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013; Vol. 3).