Association of small CAB-like proteins (SCPs) of Synechocystis sp. PCC 6803 with Photosystem II

Galyna Kufryk, Miguel A. Hernandez-Prieto, Thomas Kieselbach, Helder Miranda, Willem Vermaas, Christiane Funk

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress conditions but their exact function remains largely unknown. Recently we showed association of ScpD with light-stressed, monomeric Photosystem II in Synechocystis sp. PCC 6803 (Yao et al. J Biol Chem 282:267-276, 2007). Here we show that ScpB associates with Photosystem II at normal growth conditions. Moreover, upon introduction of a construct into Synechocystis so that ScpB is expressed continuously under normal growth conditions, ScpE was detected under non-stressed conditions as well, and was copurified with tagged ScpB and Photosystem II. We also report on a one-helix protein, Slr1544, that is somewhat similar to the SCPs and whose gene is cotranscribed with that of ScpD; Slr1544 is another member of the extended light-harvesting-like (Lil) protein family, and we propose to name it LilA.

Original languageEnglish (US)
Pages (from-to)135-145
Number of pages11
JournalPhotosynthesis research
Volume95
Issue number2-3
DOIs
StatePublished - Feb 1 2008

Keywords

  • Antenna
  • Chlorophyll-binding protein
  • Cyanobacteria
  • Early-light induced proteins (ELIPs)
  • High-light induced proteins (HLIPs)
  • Light-harvesting complex
  • Synechocystis sp. PCC 6803

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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