Article a strategy to establish a quality assurance/quality control plan for the application of biosensors for the detection of e. coli in water

Nikou Hesari, Nursel Kiratli Yilmazçoban, Mohamad Elzein, Absar Alum, Morteza Abbaszadegan

Research output: Contribution to journalArticle

Abstract

Rapid bacterial detection using biosensors is a novel approach for microbiological testing applications. Validation of such methods is an obstacle in the adoption of new bio-sensing technologies for water testing. Therefore, establishing a quality assurance and quality control (QA/QC) plan is essential to demonstrate accuracy and reliability of the biosensor method for the detection of E. coli in drinking water samples. In this study, different reagents and assay conditions including temperatures, holding time, E. coli strains and concentrations, dissolving agents, salinity and pH effects, quality of substrates of various suppliers of 4-methylumbelliferyl glucuronide (MUG), and environmental water samples were included in the QA/QC plan and used in the assay optimization and documentation. Furthermore, the procedural QA/QC for the monitoring of drinking water samples was established to validate the performance of the biosensor platform for the detection of E. coli using a culture-based standard technique. Implementing the developed QA/QC plan, the same level of precision and accuracy was achieved using both the standard and the biosensor methods. The established procedural QA/QC for the biosensor will provide a reliable tool for a near real-time monitoring of E. coli in drinking water samples to both industry and regulatory authorities.

Original languageEnglish (US)
Article number7
JournalBiosensors
Volume7
Issue number1
DOIs
StatePublished - Jan 3 2017

Keywords

  • Drinking water
  • E. coli
  • MUG substrate
  • Quality assurance/quality control
  • Rapid bacterial detection
  • Β-D-glucuronides'

ASJC Scopus subject areas

  • Clinical Biochemistry

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