Article a strategy to establish a quality assurance/quality control plan for the application of biosensors for the detection of e. coli in water

Nikou Hesari, Nursel Kiratli Yilmazçoban, Mohamad Elzein, Absar Alum, Morteza Abbaszadegan

Research output: Contribution to journalArticle

Abstract

Rapid bacterial detection using biosensors is a novel approach for microbiological testing applications. Validation of such methods is an obstacle in the adoption of new bio-sensing technologies for water testing. Therefore, establishing a quality assurance and quality control (QA/QC) plan is essential to demonstrate accuracy and reliability of the biosensor method for the detection of E. coli in drinking water samples. In this study, different reagents and assay conditions including temperatures, holding time, E. coli strains and concentrations, dissolving agents, salinity and pH effects, quality of substrates of various suppliers of 4-methylumbelliferyl glucuronide (MUG), and environmental water samples were included in the QA/QC plan and used in the assay optimization and documentation. Furthermore, the procedural QA/QC for the monitoring of drinking water samples was established to validate the performance of the biosensor platform for the detection of E. coli using a culture-based standard technique. Implementing the developed QA/QC plan, the same level of precision and accuracy was achieved using both the standard and the biosensor methods. The established procedural QA/QC for the biosensor will provide a reliable tool for a near real-time monitoring of E. coli in drinking water samples to both industry and regulatory authorities.

Original languageEnglish (US)
Article number7
JournalBiosensors
Volume7
Issue number1
DOIs
StatePublished - Jan 3 2017

Fingerprint

Biosensing Techniques
Quality assurance
Biosensors
Quality Control
Quality control
Escherichia coli
Water
Drinking Water
Assays
pH effects
Monitoring
Salinity
Testing
Documentation
Industry
Technology
Temperature
Substrates

Keywords

  • Drinking water
  • E. coli
  • MUG substrate
  • Quality assurance/quality control
  • Rapid bacterial detection
  • Β-D-glucuronides'

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Article a strategy to establish a quality assurance/quality control plan for the application of biosensors for the detection of e. coli in water. / Hesari, Nikou; Yilmazçoban, Nursel Kiratli; Elzein, Mohamad; Alum, Absar; Abbaszadegan, Morteza.

In: Biosensors, Vol. 7, No. 1, 7, 03.01.2017.

Research output: Contribution to journalArticle

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