Archaeal aminoacyl-tRNA synthesis

Diversity replaces dogma

Debra Hansen, Ute C. Vothknecht, Hyun Soo Kim, Michael Ibba, Bokkee Min, Tong Li, Joanne Pelaschier, Constantinos Stathopoulos, Hubert Becker, Dieter Söll

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Accurate aminoacyl-tRNA synthesis is essential for faithful translation of the genetic code and consequently has been intensively studied for over three decades. Until recently, the study of aminoacyl-tRNA synthesis in archaea had received little attention. However, as in so many areas of molecular biology, the advent of archaeal genome sequencing has now drawn researchers to this field. Investigations with archaea have already led to the discovery of novel pathways and enzymes for the synthesis of numerous aminoacyl-tRNAs. The most surprising of these findings has been a transamidation pathway for the synthesis of asparaginyl-tRNA and a novel lysyl-tRNA synthetase. In addition, seryl- and phenylalanyl-tRNA synthetases that are only marginally related to known examples outside the archaea have been characterized, and the mechanism of cysteinyl-tRNA formation in Methanococcus jannaschii and Methanobacterium thermoautotrophicum is still unknown. These results have revealed completely unexpected levels of complexity and diversity, questioning the notion that aminoacyl-tRNA synthesis is one of the most conserved functions in gene expression. It has now become clear that the distribution of the various mechanisms of aminoacyl-tRNA synthesis in extant organisms has been determined by numerous gene transfer events, indicating that, while the process of protein biosynthesis is orthologous, its constituents are not.

Original languageEnglish (US)
Pages (from-to)1269-1276
Number of pages8
JournalGenetics
Volume152
Issue number4
StatePublished - Aug 1 1999
Externally publishedYes

Fingerprint

Transfer RNA
Archaea
Serine-tRNA Ligase
Lysine-tRNA Ligase
Archaeal Genome
Phenylalanine-tRNA Ligase
Methanocaldococcus
Methanobacterium
Genetic Code
Protein Biosynthesis
Molecular Biology
Research Personnel
Gene Expression
Enzymes
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Hansen, D., Vothknecht, U. C., Kim, H. S., Ibba, M., Min, B., Li, T., ... Söll, D. (1999). Archaeal aminoacyl-tRNA synthesis: Diversity replaces dogma. Genetics, 152(4), 1269-1276.

Archaeal aminoacyl-tRNA synthesis : Diversity replaces dogma. / Hansen, Debra; Vothknecht, Ute C.; Kim, Hyun Soo; Ibba, Michael; Min, Bokkee; Li, Tong; Pelaschier, Joanne; Stathopoulos, Constantinos; Becker, Hubert; Söll, Dieter.

In: Genetics, Vol. 152, No. 4, 01.08.1999, p. 1269-1276.

Research output: Contribution to journalArticle

Hansen, D, Vothknecht, UC, Kim, HS, Ibba, M, Min, B, Li, T, Pelaschier, J, Stathopoulos, C, Becker, H & Söll, D 1999, 'Archaeal aminoacyl-tRNA synthesis: Diversity replaces dogma', Genetics, vol. 152, no. 4, pp. 1269-1276.
Hansen D, Vothknecht UC, Kim HS, Ibba M, Min B, Li T et al. Archaeal aminoacyl-tRNA synthesis: Diversity replaces dogma. Genetics. 1999 Aug 1;152(4):1269-1276.
Hansen, Debra ; Vothknecht, Ute C. ; Kim, Hyun Soo ; Ibba, Michael ; Min, Bokkee ; Li, Tong ; Pelaschier, Joanne ; Stathopoulos, Constantinos ; Becker, Hubert ; Söll, Dieter. / Archaeal aminoacyl-tRNA synthesis : Diversity replaces dogma. In: Genetics. 1999 ; Vol. 152, No. 4. pp. 1269-1276.
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