Application of Proteomics to Disease Diagnostics

Samir Hanash, Ji Qiu, Vitor Faca

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

An important aspect of the application of proteomics to the identification of disease biomarkers pertains to the substantial need for informatics resources. The proteomics field is now capturing the attention of computer scientists and bioinformaticians. New computational tools are being developed in several different areas, ranging from better algorithms for protein identification and measurements of statistical confidence in identification, to de novo peptide sequencing and whole genome databank searches. This chapter reviews the application of proteomics to disease diagnostics, particularly from the perspective of cancer. The emphasis is on the application of sound strategies rather than detailed description of methodologies and instrumentation. A useful repertoire of proteomics technologies is currently available for disease-related applications, though further technological innovations would be beneficial to increase sensitivity, reduce sample size requirements, increase throughput, and more effectively uncover various types of protein alterations, such as post-translational modifications. The application of high-throughput procedures to the discovery of biomarkers leads to large datasets of multi-dimensional data and a certain percentage of false discoveries. It becomes crucial to carefully design validation studies and characterize the sensitivity and specificity of each biomarker candidate in a representative population.

Original languageEnglish (US)
Title of host publicationMolecular Diagnostics: Second Edition
PublisherElsevier Ltd.
Pages285-291
Number of pages7
ISBN (Print)9780123745378
DOIs
StatePublished - Sep 2009
Externally publishedYes

Fingerprint

Proteomics
Biomarkers
Inventions
Informatics
Throughput
Validation Studies
Post Translational Protein Processing
Sample Size
Proteins
Genome
Databases
Technology
Sensitivity and Specificity
Peptides
Genes
Innovation
Acoustic waves
Population
Neoplasms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hanash, S., Qiu, J., & Faca, V. (2009). Application of Proteomics to Disease Diagnostics. In Molecular Diagnostics: Second Edition (pp. 285-291). Elsevier Ltd.. https://doi.org/10.1016/B978-0-12-374537-8.00018-3

Application of Proteomics to Disease Diagnostics. / Hanash, Samir; Qiu, Ji; Faca, Vitor.

Molecular Diagnostics: Second Edition. Elsevier Ltd., 2009. p. 285-291.

Research output: Chapter in Book/Report/Conference proceedingChapter

Hanash, S, Qiu, J & Faca, V 2009, Application of Proteomics to Disease Diagnostics. in Molecular Diagnostics: Second Edition. Elsevier Ltd., pp. 285-291. https://doi.org/10.1016/B978-0-12-374537-8.00018-3
Hanash S, Qiu J, Faca V. Application of Proteomics to Disease Diagnostics. In Molecular Diagnostics: Second Edition. Elsevier Ltd. 2009. p. 285-291 https://doi.org/10.1016/B978-0-12-374537-8.00018-3
Hanash, Samir ; Qiu, Ji ; Faca, Vitor. / Application of Proteomics to Disease Diagnostics. Molecular Diagnostics: Second Edition. Elsevier Ltd., 2009. pp. 285-291
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